Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality
RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimizati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60474/ http://psasir.upm.edu.my/id/eprint/60474/1/Optimization%20of%20illumina%20TruSeq%20Targeted%20RNA%20expression%20%28TREx%29%20library%20quality.pdf |
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| author | Abu, Nadiah Yeap, Swee Keong Nordin, Noraini Tan, Sheau Wei Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu |
| author_facet | Abu, Nadiah Yeap, Swee Keong Nordin, Noraini Tan, Sheau Wei Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu |
| author_sort | Abu, Nadiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyA-enriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA. |
| first_indexed | 2025-11-15T11:06:09Z |
| format | Article |
| id | upm-60474 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:09Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-604742018-05-21T03:51:08Z http://psasir.upm.edu.my/id/eprint/60474/ Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality Abu, Nadiah Yeap, Swee Keong Nordin, Noraini Tan, Sheau Wei Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyA-enriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60474/1/Optimization%20of%20illumina%20TruSeq%20Targeted%20RNA%20expression%20%28TREx%29%20library%20quality.pdf Abu, Nadiah and Yeap, Swee Keong and Nordin, Noraini and Tan, Sheau Wei and Ho, Wan Yong and Mohammed Alitheen, Noorjahan Banu (2018) Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality. Sains Malaysiana, 47 (2). pp. 303-308. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num2_2018/contentsVol47num2_2018.html 10.17576/jsm-2018-4702-12 |
| spellingShingle | Abu, Nadiah Yeap, Swee Keong Nordin, Noraini Tan, Sheau Wei Ho, Wan Yong Mohammed Alitheen, Noorjahan Banu Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title | Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title_full | Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title_fullStr | Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title_full_unstemmed | Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title_short | Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality |
| title_sort | optimization of illumina truseq targeted rna expression (trex) library quality |
| url | http://psasir.upm.edu.my/id/eprint/60474/ http://psasir.upm.edu.my/id/eprint/60474/ http://psasir.upm.edu.my/id/eprint/60474/ http://psasir.upm.edu.my/id/eprint/60474/1/Optimization%20of%20illumina%20TruSeq%20Targeted%20RNA%20expression%20%28TREx%29%20library%20quality.pdf |