Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products
Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride a...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60473/ http://psasir.upm.edu.my/id/eprint/60473/1/Production%20of%20phytase%20by%20Mitsuokella%20jalaludinii%20in%20semi-solid%20state%20fermentation%20of%20agricultural%20by-products.pdf |
| _version_ | 1848854204338143232 |
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| author | Tang, Hooi Chia Sieo, Chin Chin Abdullah, Norhani Mohamad, Rosfarizan Omar, Abdul Rahman Chong, Chun Wie Fitrianto, Anwar Ho, Yin Wan |
| author_facet | Tang, Hooi Chia Sieo, Chin Chin Abdullah, Norhani Mohamad, Rosfarizan Omar, Abdul Rahman Chong, Chun Wie Fitrianto, Anwar Ho, Yin Wan |
| author_sort | Tang, Hooi Chia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride and minerals) were evaluated in a one-factor-at-a-time approach. Rice bran and fish meal produced better growth and phytase enzyme activity. The removal of L-cysteine hydrochloride and minerals significantly decreased (p<0.05) phytase activity from 1178.72 U to 446.99 U and 902.54 U, respectively. The response surface methods (RSM) was conducted to optimize the phytase production and the results showed the combination of 7.7% of rice bran and 3.7% of fish meal in semi-solid state fermentation gave the highest phytase activity. Maximum phytase production and optimum growth of bacteria were detected at 12 h incubation in both MF medium (control) and agro-medium. In this agro-medium, M. jalaludinii produced 2.5 fold higher phytase activity compared to MF medium. |
| first_indexed | 2025-11-15T11:06:09Z |
| format | Article |
| id | upm-60473 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:09Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-604732018-05-21T03:51:10Z http://psasir.upm.edu.my/id/eprint/60473/ Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products Tang, Hooi Chia Sieo, Chin Chin Abdullah, Norhani Mohamad, Rosfarizan Omar, Abdul Rahman Chong, Chun Wie Fitrianto, Anwar Ho, Yin Wan Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride and minerals) were evaluated in a one-factor-at-a-time approach. Rice bran and fish meal produced better growth and phytase enzyme activity. The removal of L-cysteine hydrochloride and minerals significantly decreased (p<0.05) phytase activity from 1178.72 U to 446.99 U and 902.54 U, respectively. The response surface methods (RSM) was conducted to optimize the phytase production and the results showed the combination of 7.7% of rice bran and 3.7% of fish meal in semi-solid state fermentation gave the highest phytase activity. Maximum phytase production and optimum growth of bacteria were detected at 12 h incubation in both MF medium (control) and agro-medium. In this agro-medium, M. jalaludinii produced 2.5 fold higher phytase activity compared to MF medium. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60473/1/Production%20of%20phytase%20by%20Mitsuokella%20jalaludinii%20in%20semi-solid%20state%20fermentation%20of%20agricultural%20by-products.pdf Tang, Hooi Chia and Sieo, Chin Chin and Abdullah, Norhani and Mohamad, Rosfarizan and Omar, Abdul Rahman and Chong, Chun Wie and Fitrianto, Anwar and Ho, Yin Wan (2018) Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products. Sains Malaysiana, 47 (2). pp. 277-286. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num2_2018/contentsVol47num2_2018.html 10.17576/jsm-2018-4702-09 |
| spellingShingle | Tang, Hooi Chia Sieo, Chin Chin Abdullah, Norhani Mohamad, Rosfarizan Omar, Abdul Rahman Chong, Chun Wie Fitrianto, Anwar Ho, Yin Wan Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title | Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title_full | Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title_fullStr | Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title_full_unstemmed | Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title_short | Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| title_sort | production of phytase by mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products |
| url | http://psasir.upm.edu.my/id/eprint/60473/ http://psasir.upm.edu.my/id/eprint/60473/ http://psasir.upm.edu.my/id/eprint/60473/ http://psasir.upm.edu.my/id/eprint/60473/1/Production%20of%20phytase%20by%20Mitsuokella%20jalaludinii%20in%20semi-solid%20state%20fermentation%20of%20agricultural%20by-products.pdf |