Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine

A facile and efficient approach to prepare metal nanoparticles doped electrospun gelatin from metal salts precursors was successfully developed. The incorporation of metal ions with antimicrobial activity into electrospun gelatin (Ge-espun) is an attractive approach to control the inflammatory react...

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Main Authors: Nuge, Tamrin, Tshai, Kim Yeow, Lim, Siew Shee, Nordin, Norshariza, Hoque, Md. Enamul
Format: Article
Language:English
Published: School of Engineering, Taylor's University College 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60462/
http://psasir.upm.edu.my/id/eprint/60462/1/Preparation%20and%20characterization%20of%20CU-%2C%20FE-%2C%20AG-%2C%20ZN-%20and%20NI-%20doped%20gelatin%20nanofibers%20for%20possible%20applications%20in%20antibacterial%20nanomedicine.pdf
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author Nuge, Tamrin
Tshai, Kim Yeow
Lim, Siew Shee
Nordin, Norshariza
Hoque, Md. Enamul
author_facet Nuge, Tamrin
Tshai, Kim Yeow
Lim, Siew Shee
Nordin, Norshariza
Hoque, Md. Enamul
author_sort Nuge, Tamrin
building UPM Institutional Repository
collection Online Access
description A facile and efficient approach to prepare metal nanoparticles doped electrospun gelatin from metal salts precursors was successfully developed. The incorporation of metal ions with antimicrobial activity into electrospun gelatin (Ge-espun) is an attractive approach to control the inflammatory reaction and prevent infection in wound. In this study, metal salts precursors AgNO3, Fe(NO3)3.9H2O, Cu(NO3)2.3H2O, Zn(NO3)2.6H2O and Ni(NO3)2.6H2O were reduced to metal nanoparticles with acetic acid as solvent and reducing agent. The agglomeration of nanoparticles was inhibited by the gelatin polymer matrix. Electrospinning of both neat Ge-espun and metal-nanoparticles/Ge-espun resulted in the formation of smooth fibres with average diameters of ~280nm and ~40nm-150nm, respectively. The efficacy of metal nanoparticles/Ge-espun against bacteria commonly found on wounds was tested with different metal loading by measuring the inhibition of colony forming units. The results indicated a broad spectrum of antibacterial activity showed by Ag/Ge-espun, followed by Fe/Ge-espun and Zn/Ge-espun. An interesting finding on the efficacy of Cu/Ge-espun and Fe/Ge-espun against the Gram’s positive bacteria is worth exploring to further investigate the potential application of metal-based antibiotics against the antibiotic-resistant bacterial strains.
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spelling upm-604622018-05-21T03:51:25Z http://psasir.upm.edu.my/id/eprint/60462/ Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine Nuge, Tamrin Tshai, Kim Yeow Lim, Siew Shee Nordin, Norshariza Hoque, Md. Enamul A facile and efficient approach to prepare metal nanoparticles doped electrospun gelatin from metal salts precursors was successfully developed. The incorporation of metal ions with antimicrobial activity into electrospun gelatin (Ge-espun) is an attractive approach to control the inflammatory reaction and prevent infection in wound. In this study, metal salts precursors AgNO3, Fe(NO3)3.9H2O, Cu(NO3)2.3H2O, Zn(NO3)2.6H2O and Ni(NO3)2.6H2O were reduced to metal nanoparticles with acetic acid as solvent and reducing agent. The agglomeration of nanoparticles was inhibited by the gelatin polymer matrix. Electrospinning of both neat Ge-espun and metal-nanoparticles/Ge-espun resulted in the formation of smooth fibres with average diameters of ~280nm and ~40nm-150nm, respectively. The efficacy of metal nanoparticles/Ge-espun against bacteria commonly found on wounds was tested with different metal loading by measuring the inhibition of colony forming units. The results indicated a broad spectrum of antibacterial activity showed by Ag/Ge-espun, followed by Fe/Ge-espun and Zn/Ge-espun. An interesting finding on the efficacy of Cu/Ge-espun and Fe/Ge-espun against the Gram’s positive bacteria is worth exploring to further investigate the potential application of metal-based antibiotics against the antibiotic-resistant bacterial strains. School of Engineering, Taylor's University College 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60462/1/Preparation%20and%20characterization%20of%20CU-%2C%20FE-%2C%20AG-%2C%20ZN-%20and%20NI-%20doped%20gelatin%20nanofibers%20for%20possible%20applications%20in%20antibacterial%20nanomedicine.pdf Nuge, Tamrin and Tshai, Kim Yeow and Lim, Siew Shee and Nordin, Norshariza and Hoque, Md. Enamul (2017) Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine. Journal of Engineering Science and Technology, 12 (spec. Mar.). pp. 68-81. ISSN 1823-4690 http://jestec.taylors.edu.my/Special%20Issues%202017.htm
spellingShingle Nuge, Tamrin
Tshai, Kim Yeow
Lim, Siew Shee
Nordin, Norshariza
Hoque, Md. Enamul
Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title_full Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title_fullStr Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title_full_unstemmed Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title_short Preparation and characterization of CU-, FE-, AG-, ZN- and NI- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
title_sort preparation and characterization of cu-, fe-, ag-, zn- and ni- doped gelatin nanofibers for possible applications in antibacterial nanomedicine
url http://psasir.upm.edu.my/id/eprint/60462/
http://psasir.upm.edu.my/id/eprint/60462/
http://psasir.upm.edu.my/id/eprint/60462/1/Preparation%20and%20characterization%20of%20CU-%2C%20FE-%2C%20AG-%2C%20ZN-%20and%20NI-%20doped%20gelatin%20nanofibers%20for%20possible%20applications%20in%20antibacterial%20nanomedicine.pdf