Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology
Medium-chain acylglycerols (or glycerides) are formed of mono-, di- and triacylglycerol classes. In this study, an alternative method to produce MCA from esterifying palm oil fatty acid distillate (PFAD) with the presence of oil palm mesocarp lipase (OPML) which is a plant-sourced lipase and PFAD is...
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School of Engineering, Taylor's University College
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60453/ http://psasir.upm.edu.my/id/eprint/60453/1/Production%20of%20medium-chain%20acylglycerols%20by%20lipase%20esterification%20in%20packed%20bed%20reactor%20process%20optimization%20by%20response%20surface%20methodology.pdf |
| _version_ | 1848854198813196288 |
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| author | Mohd Dom, Zanariah Ling, Tau Chuan Yusoff, Rozita |
| author_facet | Mohd Dom, Zanariah Ling, Tau Chuan Yusoff, Rozita |
| author_sort | Mohd Dom, Zanariah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Medium-chain acylglycerols (or glycerides) are formed of mono-, di- and triacylglycerol classes. In this study, an alternative method to produce MCA from esterifying palm oil fatty acid distillate (PFAD) with the presence of oil palm mesocarp lipase (OPML) which is a plant-sourced lipase and PFAD is also cheap by-product is developed in a packed bed reactor. The production of medium-chain acylglycerols (MCA) by lipase-catalysed esterification of palm oil fatty acid distillate with glycerol are optimize in order to determine the factors that have significant effects on the reaction condition and high yield of MCA. Response surface methodology (RSM) was applied to optimize the reaction conditions. The reaction conditions, namely, the reaction time (30-240 min), enzyme load (0.5-1.5 kg), silica gel load (0.2-1.0 kg), and solvent amount (200-600 vol/wt). Reaction time, enzyme loading and solvent amount strongly effect MCA synthesis (p<0.05). However, water absorbent (silica gel) loading did not have significant (p>0.05) influence on MCA yield. Best-fitting models were successfully established for MCA yield (R2 =0.9133). The optimum MCA yield were 75% from the predicted value and 75.4% from the experimental data for 6 kg enzyme loading, a reaction time of 135min and a solvent amount of 350 vol/wt at 65°C reaction temperature. Verification of experimental results under optimized reaction conditions were conducted, and the results agreed well with the predicted range. Esterification products (mono-, di- and triacylglycerol) from the PBR were identified using Thin Layer Chromatography method. The chromatograms showed the successful fractionation of esterified products in this alternative method of process esterification. |
| first_indexed | 2025-11-15T11:06:04Z |
| format | Article |
| id | upm-60453 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:04Z |
| publishDate | 2014 |
| publisher | School of Engineering, Taylor's University College |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-604532018-05-21T03:51:33Z http://psasir.upm.edu.my/id/eprint/60453/ Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology Mohd Dom, Zanariah Ling, Tau Chuan Yusoff, Rozita Medium-chain acylglycerols (or glycerides) are formed of mono-, di- and triacylglycerol classes. In this study, an alternative method to produce MCA from esterifying palm oil fatty acid distillate (PFAD) with the presence of oil palm mesocarp lipase (OPML) which is a plant-sourced lipase and PFAD is also cheap by-product is developed in a packed bed reactor. The production of medium-chain acylglycerols (MCA) by lipase-catalysed esterification of palm oil fatty acid distillate with glycerol are optimize in order to determine the factors that have significant effects on the reaction condition and high yield of MCA. Response surface methodology (RSM) was applied to optimize the reaction conditions. The reaction conditions, namely, the reaction time (30-240 min), enzyme load (0.5-1.5 kg), silica gel load (0.2-1.0 kg), and solvent amount (200-600 vol/wt). Reaction time, enzyme loading and solvent amount strongly effect MCA synthesis (p<0.05). However, water absorbent (silica gel) loading did not have significant (p>0.05) influence on MCA yield. Best-fitting models were successfully established for MCA yield (R2 =0.9133). The optimum MCA yield were 75% from the predicted value and 75.4% from the experimental data for 6 kg enzyme loading, a reaction time of 135min and a solvent amount of 350 vol/wt at 65°C reaction temperature. Verification of experimental results under optimized reaction conditions were conducted, and the results agreed well with the predicted range. Esterification products (mono-, di- and triacylglycerol) from the PBR were identified using Thin Layer Chromatography method. The chromatograms showed the successful fractionation of esterified products in this alternative method of process esterification. School of Engineering, Taylor's University College 2014 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60453/1/Production%20of%20medium-chain%20acylglycerols%20by%20lipase%20esterification%20in%20packed%20bed%20reactor%20process%20optimization%20by%20response%20surface%20methodology.pdf Mohd Dom, Zanariah and Ling, Tau Chuan and Yusoff, Rozita (2014) Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology. Journal of Engineering Science and Technology, 9 (3). pp. 384-397. ISSN 1823-4690 http://jestec.taylors.edu.my/V9Issue3.htm |
| spellingShingle | Mohd Dom, Zanariah Ling, Tau Chuan Yusoff, Rozita Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title | Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title_full | Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title_fullStr | Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title_full_unstemmed | Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title_short | Production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| title_sort | production of medium-chain acylglycerols by lipase esterification in packed bed reactor: process optimization by response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/60453/ http://psasir.upm.edu.my/id/eprint/60453/ http://psasir.upm.edu.my/id/eprint/60453/1/Production%20of%20medium-chain%20acylglycerols%20by%20lipase%20esterification%20in%20packed%20bed%20reactor%20process%20optimization%20by%20response%20surface%20methodology.pdf |