Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology
Optimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was...
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| Format: | Article |
| Language: | English |
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Springer
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60451/ http://psasir.upm.edu.my/id/eprint/60451/1/Modeling%20and%20optimization%20of%20lipozyme%20RM%20IM-catalyzed%20esterification%20of%20medium-%20and%20long-chain%20triacyglycerols%20%28MLCT%29%20using%20response%20surface%20methodology.pdf |
| _version_ | 1848854198206070784 |
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| author | Arifin, Norlelawati Koh, Soo Peng Long, Kamariah Tan, Chin Ping Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_facet | Arifin, Norlelawati Koh, Soo Peng Long, Kamariah Tan, Chin Ping Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_sort | Arifin, Norlelawati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Optimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8–24 h), enzyme load (5–15 wt.%), and fatty acids/glycerol ratio (3:1–4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R 2 = 0.9507) and residual FFA (R 2 = 0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6–14.0 h for reaction time, 7.9–8.0 wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P > 0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production. |
| first_indexed | 2025-11-15T11:06:03Z |
| format | Article |
| id | upm-60451 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:03Z |
| publishDate | 2012 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-604512018-05-21T03:41:35Z http://psasir.upm.edu.my/id/eprint/60451/ Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology Arifin, Norlelawati Koh, Soo Peng Long, Kamariah Tan, Chin Ping Yusoff, Mohd Suria Affandi Lai, Oi Ming Optimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8–24 h), enzyme load (5–15 wt.%), and fatty acids/glycerol ratio (3:1–4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R 2 = 0.9507) and residual FFA (R 2 = 0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6–14.0 h for reaction time, 7.9–8.0 wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P > 0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production. Springer 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60451/1/Modeling%20and%20optimization%20of%20lipozyme%20RM%20IM-catalyzed%20esterification%20of%20medium-%20and%20long-chain%20triacyglycerols%20%28MLCT%29%20using%20response%20surface%20methodology.pdf Arifin, Norlelawati and Koh, Soo Peng and Long, Kamariah and Tan, Chin Ping and Yusoff, Mohd Suria Affandi and Lai, Oi Ming (2012) Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology. Food and Bioprocess Technology, 5 (1). pp. 216-225. ISSN 1935-5130; ESSN: 1935-5149 https://rd.springer.com/article/10.1007%2Fs11947-010-0325-5 10.1007/s11947-010-0325-5 |
| spellingShingle | Arifin, Norlelawati Koh, Soo Peng Long, Kamariah Tan, Chin Ping Yusoff, Mohd Suria Affandi Lai, Oi Ming Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title | Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title_full | Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title_fullStr | Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title_full_unstemmed | Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title_short | Modeling and optimization of lipozyme RM IM-catalyzed esterification of medium- and long-chain triacyglycerols (MLCT) using response surface methodology |
| title_sort | modeling and optimization of lipozyme rm im-catalyzed esterification of medium- and long-chain triacyglycerols (mlct) using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/60451/ http://psasir.upm.edu.my/id/eprint/60451/ http://psasir.upm.edu.my/id/eprint/60451/ http://psasir.upm.edu.my/id/eprint/60451/1/Modeling%20and%20optimization%20of%20lipozyme%20RM%20IM-catalyzed%20esterification%20of%20medium-%20and%20long-chain%20triacyglycerols%20%28MLCT%29%20using%20response%20surface%20methodology.pdf |