Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia
Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogen...
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| Format: | Article |
| Language: | English English |
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Springer Science+Business Media, LLC 2009
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5985/ http://psasir.upm.edu.my/id/eprint/5985/1/Sequence%20and%20phylogenetic%20analysis%20of%20S1.pdf |
| _version_ | 1848840250927874048 |
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| author | Mohamed Zulperi, Zarirah Omar, Abdul Rahman Arshad, Siti Suri |
| author_facet | Mohamed Zulperi, Zarirah Omar, Abdul Rahman Arshad, Siti Suri |
| author_sort | Mohamed Zulperi, Zarirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region
of both IBV isolates. Phylogenetic analysis of S1 gene and
sequences analysis of M gene indicated that MH5365/95
and V9/04 belong to non-Massachusetts strain. However,
both isolates share only 77% identity. Analysis based on S1
gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67–77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other,
and there are fewer mutations which occurred in the
respective genes. |
| first_indexed | 2025-11-15T07:24:22Z |
| format | Article |
| id | upm-5985 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:24:22Z |
| publishDate | 2009 |
| publisher | Springer Science+Business Media, LLC 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-59852015-10-22T03:35:35Z http://psasir.upm.edu.my/id/eprint/5985/ Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia Mohamed Zulperi, Zarirah Omar, Abdul Rahman Arshad, Siti Suri Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogenetic analysis of S1 gene and sequences analysis of M gene indicated that MH5365/95 and V9/04 belong to non-Massachusetts strain. However, both isolates share only 77% identity. Analysis based on S1 gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67–77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other, and there are fewer mutations which occurred in the respective genes. Springer Science+Business Media, LLC 2009 2009-02-26 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5985/1/Sequence%20and%20phylogenetic%20analysis%20of%20S1.pdf Mohamed Zulperi, Zarirah and Omar, Abdul Rahman and Arshad, Siti Suri (2009) Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia. Virus Genes, 38 (3). pp. 383-391. ISSN 0920-8569 http://dx.doi.org/10.1007/s11262-009-0337-2 10.1007/s11262-009-0337-2 English |
| spellingShingle | Mohamed Zulperi, Zarirah Omar, Abdul Rahman Arshad, Siti Suri Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia |
| title | Sequence and phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia |
| title_full | Sequence and phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia |
| title_fullStr | Sequence and phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia |
| title_full_unstemmed | Sequence and phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia |
| title_short | Sequence and phylogenetic analysis of S1, S2, M, and N genes
of infectious bronchitis virus isolates from Malaysia |
| title_sort | sequence and phylogenetic analysis of s1, s2, m, and n genes
of infectious bronchitis virus isolates from malaysia |
| url | http://psasir.upm.edu.my/id/eprint/5985/ http://psasir.upm.edu.my/id/eprint/5985/ http://psasir.upm.edu.my/id/eprint/5985/ http://psasir.upm.edu.my/id/eprint/5985/1/Sequence%20and%20phylogenetic%20analysis%20of%20S1.pdf |