Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia
Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Science+Business Media, LLC 2009
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5985/ http://psasir.upm.edu.my/id/eprint/5985/1/Sequence%20and%20phylogenetic%20analysis%20of%20S1.pdf |
| Summary: | Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region
of both IBV isolates. Phylogenetic analysis of S1 gene and
sequences analysis of M gene indicated that MH5365/95
and V9/04 belong to non-Massachusetts strain. However,
both isolates share only 77% identity. Analysis based on S1
gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67–77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other,
and there are fewer mutations which occurred in the
respective genes. |
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