Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA
Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element wer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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International Union of Biochemistry and Molecular Biology
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/59688/ http://psasir.upm.edu.my/id/eprint/59688/1/Wide%20dynamic%20range%20of%20surface-plasmon-resonance-based%20assay%20for%20hepatitis%20B%20surface%20antigen%20antibody%20optimal%20detection%20in%20comparison%20with%20ELISA.pdf |
| _version_ | 1848853991181516800 |
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| author | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mustafa, Nor Hidayah Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Abdullah, Rasedee |
| author_facet | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mustafa, Nor Hidayah Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Abdullah, Rasedee |
| author_sort | Tam, Yew Joon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element were established through the scouting for optimal conditions for the improvement of immobilization efficiency and in the use of optimal regeneration buffer. Recombinant HBsAg was immobilized onto the sensor surface of a CM5 chip at a concentration of 150 mg/L in sodium acetate buffer at pH 4 with added 0.6% Triton X-100. A regeneration solution of 20 mM HCl was optimally found to effectively unbind analytes from the ligand, thus allowing for multiple screening cycles. A dynamic range of detection of ∼0.00098–0.25 mg/L was obtained, and a sevenfold higher LOD, as well as a twofold increase in coefficient of variance of the replicated results, was shown as compared with enzyme-linked immunosorbent assay (ELISA). Evaluation of the assay for specificity showed no cross-reactivity with other antibodies tested. The ability of SPR chip-based assay and ELISA to detect anti-HBs in human serum was comparable, indicating that the SPR chip-based assay with its multiple screening capacity has greater advantage over ELISA. |
| first_indexed | 2025-11-15T11:02:46Z |
| format | Article |
| id | upm-59688 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:02:46Z |
| publishDate | 2017 |
| publisher | International Union of Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-596882018-03-16T07:21:35Z http://psasir.upm.edu.my/id/eprint/59688/ Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mustafa, Nor Hidayah Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Abdullah, Rasedee Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element were established through the scouting for optimal conditions for the improvement of immobilization efficiency and in the use of optimal regeneration buffer. Recombinant HBsAg was immobilized onto the sensor surface of a CM5 chip at a concentration of 150 mg/L in sodium acetate buffer at pH 4 with added 0.6% Triton X-100. A regeneration solution of 20 mM HCl was optimally found to effectively unbind analytes from the ligand, thus allowing for multiple screening cycles. A dynamic range of detection of ∼0.00098–0.25 mg/L was obtained, and a sevenfold higher LOD, as well as a twofold increase in coefficient of variance of the replicated results, was shown as compared with enzyme-linked immunosorbent assay (ELISA). Evaluation of the assay for specificity showed no cross-reactivity with other antibodies tested. The ability of SPR chip-based assay and ELISA to detect anti-HBs in human serum was comparable, indicating that the SPR chip-based assay with its multiple screening capacity has greater advantage over ELISA. International Union of Biochemistry and Molecular Biology 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59688/1/Wide%20dynamic%20range%20of%20surface-plasmon-resonance-based%20assay%20for%20hepatitis%20B%20surface%20antigen%20antibody%20optimal%20detection%20in%20comparison%20with%20ELISA.pdf Tam, Yew Joon and Allaudin, Zeenathul Nazariah and Rezaei, Morvarid Akhavan and Mustafa, Nor Hidayah and Mohd Lila, Mohd Azmi and Bahaman, Abdul Rani and Lo, Sewn Cen and Tan, Joo Shun and Hani, Homayoun and Abdullah, Rasedee (2017) Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA. Biotechnology and Applied Biochemistry, 64 (5). pp. 735-744. ISSN 0885-4513; ESSN: 1470-8744 http://onlinelibrary.wiley.com/wol1/doi/10.1002/bab.1528/abstract 10.1002/bab.1528 |
| spellingShingle | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mustafa, Nor Hidayah Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Abdullah, Rasedee Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title | Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title_full | Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title_fullStr | Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title_full_unstemmed | Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title_short | Wide dynamic range of surface-plasmon-resonance-based assay for hepatitis B surface antigen antibody optimal detection in comparison with ELISA |
| title_sort | wide dynamic range of surface-plasmon-resonance-based assay for hepatitis b surface antigen antibody optimal detection in comparison with elisa |
| url | http://psasir.upm.edu.my/id/eprint/59688/ http://psasir.upm.edu.my/id/eprint/59688/ http://psasir.upm.edu.my/id/eprint/59688/ http://psasir.upm.edu.my/id/eprint/59688/1/Wide%20dynamic%20range%20of%20surface-plasmon-resonance-based%20assay%20for%20hepatitis%20B%20surface%20antigen%20antibody%20optimal%20detection%20in%20comparison%20with%20ELISA.pdf |