Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences
To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four du...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/59609/ http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf |
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| author | Siew, Ging Yang Ng, Wei Lun Salleh, Muhammad Fadzly Tan, Sheau Wei Ky, Huynh Mohammed Alitheen, Noorjahan Banu Tan, Soon Guan Yeap, Swee Keong |
| author_facet | Siew, Ging Yang Ng, Wei Lun Salleh, Muhammad Fadzly Tan, Sheau Wei Ky, Huynh Mohammed Alitheen, Noorjahan Banu Tan, Soon Guan Yeap, Swee Keong |
| author_sort | Siew, Ging Yang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties. |
| first_indexed | 2025-11-15T11:02:25Z |
| format | Article |
| id | upm-59609 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:02:25Z |
| publishDate | 2018 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-596092018-03-13T00:44:09Z http://psasir.upm.edu.my/id/eprint/59609/ Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences Siew, Ging Yang Ng, Wei Lun Salleh, Muhammad Fadzly Tan, Sheau Wei Ky, Huynh Mohammed Alitheen, Noorjahan Banu Tan, Soon Guan Yeap, Swee Keong To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties. Universiti Putra Malaysia Press 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf Siew, Ging Yang and Ng, Wei Lun and Salleh, Muhammad Fadzly and Tan, Sheau Wei and Ky, Huynh and Mohammed Alitheen, Noorjahan Banu and Tan, Soon Guan and Yeap, Swee Keong (2018) Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences. Pertanika Journal of Tropical Agricultural Science, 41 (1). pp. 321-332. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2041%20(1)%20Feb.%202018/21%20JTAS-1110-2017-5thProof.pdf |
| spellingShingle | Siew, Ging Yang Ng, Wei Lun Salleh, Muhammad Fadzly Tan, Sheau Wei Ky, Huynh Mohammed Alitheen, Noorjahan Banu Tan, Soon Guan Yeap, Swee Keong Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title | Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title_full | Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title_fullStr | Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title_full_unstemmed | Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title_short | Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences |
| title_sort | assessment of the genetic variation of malaysian durian varieties using inter-simple sequence repeat markers and chloroplast dna sequences |
| url | http://psasir.upm.edu.my/id/eprint/59609/ http://psasir.upm.edu.my/id/eprint/59609/ http://psasir.upm.edu.my/id/eprint/59609/1/21%20JTAS-1110-2017-5thProof.pdf |