Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense

Basal stem rot (BSR) caused by Ganoderma boninense, a white-rot fungus is one of the major threats in oil palm industry that accounted for huge economic losses. Furthermore, the defense mechanism of oil palm against G. boninense infection is poorly understood. Elucidation of the molecular changes in...

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Main Authors: Abdul Bahari, Mohammad Nazri, Mohd Sakeh, Nurshafika, Abdullah, Siti Nor Akmar, Ramli, Redzyque Ramza, Kadkhodaei, Saied
Format: Conference or Workshop Item
Language:English
Published: Institute of Plantation Studies, Universiti Putra Malaysia 2017
Online Access:http://psasir.upm.edu.my/id/eprint/58860/
http://psasir.upm.edu.my/id/eprint/58860/1/Technical_Paper_3.pdf
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author Abdul Bahari, Mohammad Nazri
Mohd Sakeh, Nurshafika
Abdullah, Siti Nor Akmar
Ramli, Redzyque Ramza
Kadkhodaei, Saied
author_facet Abdul Bahari, Mohammad Nazri
Mohd Sakeh, Nurshafika
Abdullah, Siti Nor Akmar
Ramli, Redzyque Ramza
Kadkhodaei, Saied
author_sort Abdul Bahari, Mohammad Nazri
building UPM Institutional Repository
collection Online Access
description Basal stem rot (BSR) caused by Ganoderma boninense, a white-rot fungus is one of the major threats in oil palm industry that accounted for huge economic losses. Furthermore, the defense mechanism of oil palm against G. boninense infection is poorly understood. Elucidation of the molecular changes in defense response in oil palm at early stages of the fungal colonization where symptoms have not yet appeared will promise further insights for prevention measures. Whole transcriptome RNA sequencing (RNA-Seq) was performed on G. boninense-infected oil palm seedlings at 3, 7, and 11 days post inoculation (d.p.i.). Analysis on differentially expressed genes (DEGs) revealed strengthening of the plant cell wall as well as degradation of fungal cell wall through chitinase activity. Flavonoids were suggested to be synthesized at later stage (7 and 11 d.p.i). Besides, 1-aminocyclopropane-1-carboxylate synthase (involves in ethylene biosynthesis) and MYC2 transcription factor genes were observed significantly induced at from 3 – 11 d.p.i. Both of the genes presumably work in concert at early interactions between oil palm and G. boninense. This study further clarifies the roles of defense response genes in oil palm at early stages of interaction with G. boninense.
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language English
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spelling upm-588602018-02-07T05:55:54Z http://psasir.upm.edu.my/id/eprint/58860/ Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense Abdul Bahari, Mohammad Nazri Mohd Sakeh, Nurshafika Abdullah, Siti Nor Akmar Ramli, Redzyque Ramza Kadkhodaei, Saied Basal stem rot (BSR) caused by Ganoderma boninense, a white-rot fungus is one of the major threats in oil palm industry that accounted for huge economic losses. Furthermore, the defense mechanism of oil palm against G. boninense infection is poorly understood. Elucidation of the molecular changes in defense response in oil palm at early stages of the fungal colonization where symptoms have not yet appeared will promise further insights for prevention measures. Whole transcriptome RNA sequencing (RNA-Seq) was performed on G. boninense-infected oil palm seedlings at 3, 7, and 11 days post inoculation (d.p.i.). Analysis on differentially expressed genes (DEGs) revealed strengthening of the plant cell wall as well as degradation of fungal cell wall through chitinase activity. Flavonoids were suggested to be synthesized at later stage (7 and 11 d.p.i). Besides, 1-aminocyclopropane-1-carboxylate synthase (involves in ethylene biosynthesis) and MYC2 transcription factor genes were observed significantly induced at from 3 – 11 d.p.i. Both of the genes presumably work in concert at early interactions between oil palm and G. boninense. This study further clarifies the roles of defense response genes in oil palm at early stages of interaction with G. boninense. Institute of Plantation Studies, Universiti Putra Malaysia 2017 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58860/1/Technical_Paper_3.pdf Abdul Bahari, Mohammad Nazri and Mohd Sakeh, Nurshafika and Abdullah, Siti Nor Akmar and Ramli, Redzyque Ramza and Kadkhodaei, Saied (2017) Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense. In: International Conference on Big Data Applications in Agriculture (ICBAA2017), 5-6 Dec. 2017, Auditorium Putra, TNCPI Building, Universiti Putra Malaysia. (pp. 86-90). http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20171231215317ICBAA2017_Technical_Paper_3.pdf
spellingShingle Abdul Bahari, Mohammad Nazri
Mohd Sakeh, Nurshafika
Abdullah, Siti Nor Akmar
Ramli, Redzyque Ramza
Kadkhodaei, Saied
Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title_full Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title_fullStr Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title_full_unstemmed Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title_short Transcriptional regulatory mechanism of early defense response of oil palm against Ganoderma boninense
title_sort transcriptional regulatory mechanism of early defense response of oil palm against ganoderma boninense
url http://psasir.upm.edu.my/id/eprint/58860/
http://psasir.upm.edu.my/id/eprint/58860/
http://psasir.upm.edu.my/id/eprint/58860/1/Technical_Paper_3.pdf