Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produ...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Institute of Plantation Studies, Universiti Putra Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58843/ http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf |
| _version_ | 1848853757895376896 |
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| author | Karim, Mohd Shahrul Nizwanshah Abdullah, Siti Nor Akmar |
| author_facet | Karim, Mohd Shahrul Nizwanshah Abdullah, Siti Nor Akmar |
| author_sort | Karim, Mohd Shahrul Nizwanshah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes. |
| first_indexed | 2025-11-15T10:59:03Z |
| format | Conference or Workshop Item |
| id | upm-58843 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:59:03Z |
| publishDate | 2017 |
| publisher | Institute of Plantation Studies, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-588432018-02-07T03:40:32Z http://psasir.upm.edu.my/id/eprint/58843/ Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension Karim, Mohd Shahrul Nizwanshah Abdullah, Siti Nor Akmar PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes. Institute of Plantation Studies, Universiti Putra Malaysia 2017 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf Karim, Mohd Shahrul Nizwanshah and Abdullah, Siti Nor Akmar (2017) Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension. In: International Conference on Big Data Applications in Agriculture (ICBAA2017), 5-6 Dec. 2017, Auditorium Putra, TNCPI Building, Universiti Putra Malaysia. (pp. 191-195). http://spel2.upm.edu.my/webupm/upload/dokumen/penerbitan/20180102001241ICBAA2017_Poster_Paper_21.pdf |
| spellingShingle | Karim, Mohd Shahrul Nizwanshah Abdullah, Siti Nor Akmar Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title | Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title_full | Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title_fullStr | Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title_full_unstemmed | Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title_short | Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension |
| title_sort | mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (hggt) by pcr-driven overlap extension |
| url | http://psasir.upm.edu.my/id/eprint/58843/ http://psasir.upm.edu.my/id/eprint/58843/ http://psasir.upm.edu.my/id/eprint/58843/1/Poster_Paper_21.pdf |