Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP)
The classification of turfgrass cultivars is difficult as it has mostly relied on morphological characteristics that are multigenic, quantitative, and susceptible to modification by environmental factors. In this study, we employed an Amplified Fragment Length Polymorphic (AFLP) technique to disting...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58530/ http://psasir.upm.edu.my/id/eprint/58530/1/Variation%20in%20turfgrasses%20demonstrated%20by%20amplified%20fragment%20length%20polymorphism%20%28AFLP%29.pdf |
| _version_ | 1848853672634613760 |
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| author | Ho, Chai Ling Kulaveerasingam, Harikrishna Kandasamy, Kodi Isparan Chin, Chiew Foan Abdul Rahman, Siti Suhaila |
| author_facet | Ho, Chai Ling Kulaveerasingam, Harikrishna Kandasamy, Kodi Isparan Chin, Chiew Foan Abdul Rahman, Siti Suhaila |
| author_sort | Ho, Chai Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The classification of turfgrass cultivars is difficult as it has mostly relied on morphological characteristics that are multigenic, quantitative, and susceptible to modification by environmental factors. In this study, we employed an Amplified Fragment Length Polymorphic (AFLP) technique to distinguish between several turfgrass samples (including two ‘offtypes’) that were collected from Malaysian golf courses in comparison to four Australian Cynodon samples. The results clearly demonstrated the applicability of AFLP as a method to identify and fingerprint turfgrass samples and their ‘offtypes’. The agreement of the three dendrograms generated by AFLP, Arbitrary Primed Polymorphic DNA (AP-PCR) and Internal Transcribed Spacer (ITS) gives good confidence in the use of the AFLP data for identification and analysis of relationships between the Australian Cynodon samples. The occurrence of ‘offtypes’ at one of the Malaysian golf courses is possibly a result from somatic mutation (TD6) or sod contamination at planting (TW4). |
| first_indexed | 2025-11-15T10:57:42Z |
| format | Article |
| id | upm-58530 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:57:42Z |
| publishDate | 2003 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-585302018-01-22T06:02:36Z http://psasir.upm.edu.my/id/eprint/58530/ Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) Ho, Chai Ling Kulaveerasingam, Harikrishna Kandasamy, Kodi Isparan Chin, Chiew Foan Abdul Rahman, Siti Suhaila The classification of turfgrass cultivars is difficult as it has mostly relied on morphological characteristics that are multigenic, quantitative, and susceptible to modification by environmental factors. In this study, we employed an Amplified Fragment Length Polymorphic (AFLP) technique to distinguish between several turfgrass samples (including two ‘offtypes’) that were collected from Malaysian golf courses in comparison to four Australian Cynodon samples. The results clearly demonstrated the applicability of AFLP as a method to identify and fingerprint turfgrass samples and their ‘offtypes’. The agreement of the three dendrograms generated by AFLP, Arbitrary Primed Polymorphic DNA (AP-PCR) and Internal Transcribed Spacer (ITS) gives good confidence in the use of the AFLP data for identification and analysis of relationships between the Australian Cynodon samples. The occurrence of ‘offtypes’ at one of the Malaysian golf courses is possibly a result from somatic mutation (TD6) or sod contamination at planting (TW4). Malaysian Society for Molecular Biology and Biotechnology 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58530/1/Variation%20in%20turfgrasses%20demonstrated%20by%20amplified%20fragment%20length%20polymorphism%20%28AFLP%29.pdf Ho, Chai Ling and Kulaveerasingam, Harikrishna and Kandasamy, Kodi Isparan and Chin, Chiew Foan and Abdul Rahman, Siti Suhaila (2003) Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP). Asia Pacific Journal of Molecular Biology and Biotechnology, 11 (1). pp. 51-55. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html111/111.htm |
| spellingShingle | Ho, Chai Ling Kulaveerasingam, Harikrishna Kandasamy, Kodi Isparan Chin, Chiew Foan Abdul Rahman, Siti Suhaila Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title | Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title_full | Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title_fullStr | Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title_full_unstemmed | Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title_short | Variation in turfgrasses demonstrated by amplified fragment length polymorphism (AFLP) |
| title_sort | variation in turfgrasses demonstrated by amplified fragment length polymorphism (aflp) |
| url | http://psasir.upm.edu.my/id/eprint/58530/ http://psasir.upm.edu.my/id/eprint/58530/ http://psasir.upm.edu.my/id/eprint/58530/1/Variation%20in%20turfgrasses%20demonstrated%20by%20amplified%20fragment%20length%20polymorphism%20%28AFLP%29.pdf |