Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD
We present an approach to profile the expression of gene family members during somatic embryogenesis of Axonopus compressus through the modification of a recently developed RNA fingerprinting method. The protocol combines restriction fragment differential display technology with primers directed to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58524/ http://psasir.upm.edu.my/id/eprint/58524/1/Identification%20of%20differentially%20expressed%20genes%20during%20somatic%20embryogenesis%20of%20Axonopus%20compressus%20by%20restriction%20fragment%20differential%20display-coupled%20FSD.pdf |
| _version_ | 1848853671051264000 |
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| author | See, Pao Theen Abdul Rahman, Siti Suhaila Chin, Chiew Foan Kulaveerasingam, Harikrishna |
| author_facet | See, Pao Theen Abdul Rahman, Siti Suhaila Chin, Chiew Foan Kulaveerasingam, Harikrishna |
| author_sort | See, Pao Theen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We present an approach to profile the expression of gene family members during somatic embryogenesis of Axonopus compressus through the modification of a recently developed RNA fingerprinting method. The protocol combines restriction fragment differential display technology with primers directed to a gene family specific domain signature on mRNA and hence is termed ‘RFDD-coupled FSD’. By using this method, two differentially expressed mRNAs, AC1 and AC2, containing the chromo-like domain and the serine/threonine protein kinase signatures, respectively, were isolated. Sequence analysis revealed that AC1encoded a MIP (plasma membrane protein) meanwhile AC2 shared low homology to other serine/threonine protein kinases. The abundance of these two transcripts appeared to be down-regulated in the embryogenic and non-viable embryogenic tissues when examined on Northern and semi-quantitative RT-PCR analyses, suggesting possible developmental arrest. This preliminary differential expression examination has somewhat provided a first insight into the molecular events underlying A. compressus somatic embryo development. Thus, RFDD-coupled FSD has been shown to be an efficient procedure for identifying differential regulated transcripts that correspond to their defined gene family domain signature sequences. |
| first_indexed | 2025-11-15T10:57:40Z |
| format | Article |
| id | upm-58524 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:57:40Z |
| publishDate | 2003 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-585242018-01-22T02:38:53Z http://psasir.upm.edu.my/id/eprint/58524/ Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD See, Pao Theen Abdul Rahman, Siti Suhaila Chin, Chiew Foan Kulaveerasingam, Harikrishna We present an approach to profile the expression of gene family members during somatic embryogenesis of Axonopus compressus through the modification of a recently developed RNA fingerprinting method. The protocol combines restriction fragment differential display technology with primers directed to a gene family specific domain signature on mRNA and hence is termed ‘RFDD-coupled FSD’. By using this method, two differentially expressed mRNAs, AC1 and AC2, containing the chromo-like domain and the serine/threonine protein kinase signatures, respectively, were isolated. Sequence analysis revealed that AC1encoded a MIP (plasma membrane protein) meanwhile AC2 shared low homology to other serine/threonine protein kinases. The abundance of these two transcripts appeared to be down-regulated in the embryogenic and non-viable embryogenic tissues when examined on Northern and semi-quantitative RT-PCR analyses, suggesting possible developmental arrest. This preliminary differential expression examination has somewhat provided a first insight into the molecular events underlying A. compressus somatic embryo development. Thus, RFDD-coupled FSD has been shown to be an efficient procedure for identifying differential regulated transcripts that correspond to their defined gene family domain signature sequences. Malaysian Society for Molecular Biology and Biotechnology 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58524/1/Identification%20of%20differentially%20expressed%20genes%20during%20somatic%20embryogenesis%20of%20Axonopus%20compressus%20by%20restriction%20fragment%20differential%20display-coupled%20FSD.pdf See, Pao Theen and Abdul Rahman, Siti Suhaila and Chin, Chiew Foan and Kulaveerasingam, Harikrishna (2003) Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD. Asia Pacific Journal of Molecular Biology and Biotechnology, 11 (2). pp. 71-76. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html112/112.htm |
| spellingShingle | See, Pao Theen Abdul Rahman, Siti Suhaila Chin, Chiew Foan Kulaveerasingam, Harikrishna Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title | Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title_full | Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title_fullStr | Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title_full_unstemmed | Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title_short | Identification of differentially expressed genes during somatic embryogenesis of Axonopus compressus by restriction fragment differential display-coupled FSD |
| title_sort | identification of differentially expressed genes during somatic embryogenesis of axonopus compressus by restriction fragment differential display-coupled fsd |
| url | http://psasir.upm.edu.my/id/eprint/58524/ http://psasir.upm.edu.my/id/eprint/58524/ http://psasir.upm.edu.my/id/eprint/58524/1/Identification%20of%20differentially%20expressed%20genes%20during%20somatic%20embryogenesis%20of%20Axonopus%20compressus%20by%20restriction%20fragment%20differential%20display-coupled%20FSD.pdf |