The operation performance of an expanded bed contactor characterised by mechanical stirring
The performance of an expanded bed contactor (UpFront i.d. 20 mm) characterised by mechanical stirring flow distribution in the adsorption of intracellular proteins from concentrated unclarified yeast extract was investigated. High density pellicular adsorbent (UpFront steel-agarose; ρ = 2.65 g ml-1...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58496/ http://psasir.upm.edu.my/id/eprint/58496/1/The%20operation%20performance%20of%20an%20expanded%20bed%20contactor%20characterised%20by%20mechanical%20stirring.pdf |
| _version_ | 1848853663736397824 |
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| author | Ling, Tau Chuan Lan, John Chi Wei Lyddiatt, Andrew |
| author_facet | Ling, Tau Chuan Lan, John Chi Wei Lyddiatt, Andrew |
| author_sort | Ling, Tau Chuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The performance of an expanded bed contactor (UpFront i.d. 20 mm) characterised by mechanical stirring flow distribution in the adsorption of intracellular proteins from concentrated unclarified yeast extract was investigated. High density pellicular adsorbent (UpFront steel-agarose; ρ = 2.65 g ml-1) derivatized with selective ligand chemistries (Cibacron Blue 3GA) was adopted in this study. The adsorption of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from bakers’ yeast was chosen as a demonstration of this approach. It was demonstrated that a high biomass throughput adsorption operation (25 % ww/v of yeast extract) was achieved in this contactor design. |
| first_indexed | 2025-11-15T10:57:33Z |
| format | Article |
| id | upm-58496 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:57:33Z |
| publishDate | 2006 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-584962018-01-18T10:15:53Z http://psasir.upm.edu.my/id/eprint/58496/ The operation performance of an expanded bed contactor characterised by mechanical stirring Ling, Tau Chuan Lan, John Chi Wei Lyddiatt, Andrew The performance of an expanded bed contactor (UpFront i.d. 20 mm) characterised by mechanical stirring flow distribution in the adsorption of intracellular proteins from concentrated unclarified yeast extract was investigated. High density pellicular adsorbent (UpFront steel-agarose; ρ = 2.65 g ml-1) derivatized with selective ligand chemistries (Cibacron Blue 3GA) was adopted in this study. The adsorption of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from bakers’ yeast was chosen as a demonstration of this approach. It was demonstrated that a high biomass throughput adsorption operation (25 % ww/v of yeast extract) was achieved in this contactor design. Malaysian Society for Molecular Biology and Biotechnology 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58496/1/The%20operation%20performance%20of%20an%20expanded%20bed%20contactor%20characterised%20by%20mechanical%20stirring.pdf Ling, Tau Chuan and Lan, John Chi Wei and Lyddiatt, Andrew (2006) The operation performance of an expanded bed contactor characterised by mechanical stirring. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 27-31. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html141/141d.htm |
| spellingShingle | Ling, Tau Chuan Lan, John Chi Wei Lyddiatt, Andrew The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title | The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title_full | The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title_fullStr | The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title_full_unstemmed | The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title_short | The operation performance of an expanded bed contactor characterised by mechanical stirring |
| title_sort | operation performance of an expanded bed contactor characterised by mechanical stirring |
| url | http://psasir.upm.edu.my/id/eprint/58496/ http://psasir.upm.edu.my/id/eprint/58496/ http://psasir.upm.edu.my/id/eprint/58496/1/The%20operation%20performance%20of%20an%20expanded%20bed%20contactor%20characterised%20by%20mechanical%20stirring.pdf |