Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato
A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58494/ http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf |
| _version_ | 1848853663190089728 |
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| author | Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna |
| author_facet | Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna |
| author_sort | Chan, Pick Kuen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of the temporal and spatial patterns of gene expression through northern blotting has demonstrated the highest level of expression in mature tomato pistils. In situ hybridization revealed that the mRNA was maximal on the upper half of the style and decreased in a gradient from the top to the lower half of the style. Hybridization was also detected on the stigma, ovules and ovary walls. The LN2-1-1 could possibly play a role in modulating the defense mechanism of tomato flowers based on its ability to bind to the chitinase, Chi 2;1 promoter. |
| first_indexed | 2025-11-15T10:57:33Z |
| format | Article |
| id | upm-58494 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:57:33Z |
| publishDate | 2006 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-584942018-01-18T10:08:49Z http://psasir.upm.edu.my/id/eprint/58494/ Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of the temporal and spatial patterns of gene expression through northern blotting has demonstrated the highest level of expression in mature tomato pistils. In situ hybridization revealed that the mRNA was maximal on the upper half of the style and decreased in a gradient from the top to the lower half of the style. Hybridization was also detected on the stigma, ovules and ovary walls. The LN2-1-1 could possibly play a role in modulating the defense mechanism of tomato flowers based on its ability to bind to the chitinase, Chi 2;1 promoter. Malaysian Society for Molecular Biology and Biotechnology 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf Chan, Pick Kuen and Mohd Yusuf, Hirzun and Wong, Clemente Michael Vui Ling and Kulaveerasingam, Harikrishna (2006) Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 19-26. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html141/141c.htm |
| spellingShingle | Chan, Pick Kuen Mohd Yusuf, Hirzun Wong, Clemente Michael Vui Ling Kulaveerasingam, Harikrishna Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title | Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title_full | Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title_fullStr | Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title_full_unstemmed | Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title_short | Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato |
| title_sort | isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, chi2;1 promoter of tomato |
| url | http://psasir.upm.edu.my/id/eprint/58494/ http://psasir.upm.edu.my/id/eprint/58494/ http://psasir.upm.edu.my/id/eprint/58494/1/Isolation%20and%20characterization%20of%20a%20novel%20pistil%20predominant%20gene%20that%20binds%20weakly%20to%20the%20chitinase.pdf |