Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat
Salmonellosis is an important public health problem and causes large economic losses in the poultry industry. The emergence of molecular technology has opened various possibilities for constructing tailor-made proteins, particularly protein E from bacteriophage PhiX174 for the production of bacteria...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/58375/ http://psasir.upm.edu.my/id/eprint/58375/1/%2862%29.pdf |
| _version_ | 1848853628857614336 |
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| author | Thung, Tze Young Chin, Yih Zhet Mohd Shahril, Najwa Anyi, Ubong New, Chia Yeung Bahumaish, Ramzi Othman Saeed Kuan, Chee Hao Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son |
| author_facet | Thung, Tze Young Chin, Yih Zhet Mohd Shahril, Najwa Anyi, Ubong New, Chia Yeung Bahumaish, Ramzi Othman Saeed Kuan, Chee Hao Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son |
| author_sort | Thung, Tze Young |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Salmonellosis is an important public health problem and causes large economic losses in the poultry industry. The emergence of molecular technology has opened various possibilities for constructing tailor-made proteins, particularly protein E from bacteriophage PhiX174 for the production of bacterial ghosts (BGs) applied in vaccines purposes. In the present study, the plamdaPRcI-Elysis plasmid carrying the PhiX174 lysis gene E and thermo-sensitive lamda PR-cl857 regulatory system was constructed. Two Salmonella Enteritidis (SE-2 and SE-4) and one Salmonella Typhimurium (ST-4) isolates were able to uptake the lysis plasmid via electrotransformation. Generation of ghosts was enhanced by increasing the incubation temperature up to 42 ̊C. Cell viability of SE-2, SE-4 and ST-4 decreased ranging in log 2.7 to log 4.1 cycles after lysis induction. Moreover, SE-2 and SE-4 exhibited the earliest reduction of CFU after 3 h of incubation. Our results may provide a promising avenue for the development of Salmonella BGs vaccines. |
| first_indexed | 2025-11-15T10:57:00Z |
| format | Article |
| id | upm-58375 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:57:00Z |
| publishDate | 2017 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-583752018-01-15T02:42:16Z http://psasir.upm.edu.my/id/eprint/58375/ Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat Thung, Tze Young Chin, Yih Zhet Mohd Shahril, Najwa Anyi, Ubong New, Chia Yeung Bahumaish, Ramzi Othman Saeed Kuan, Chee Hao Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son Salmonellosis is an important public health problem and causes large economic losses in the poultry industry. The emergence of molecular technology has opened various possibilities for constructing tailor-made proteins, particularly protein E from bacteriophage PhiX174 for the production of bacterial ghosts (BGs) applied in vaccines purposes. In the present study, the plamdaPRcI-Elysis plasmid carrying the PhiX174 lysis gene E and thermo-sensitive lamda PR-cl857 regulatory system was constructed. Two Salmonella Enteritidis (SE-2 and SE-4) and one Salmonella Typhimurium (ST-4) isolates were able to uptake the lysis plasmid via electrotransformation. Generation of ghosts was enhanced by increasing the incubation temperature up to 42 ̊C. Cell viability of SE-2, SE-4 and ST-4 decreased ranging in log 2.7 to log 4.1 cycles after lysis induction. Moreover, SE-2 and SE-4 exhibited the earliest reduction of CFU after 3 h of incubation. Our results may provide a promising avenue for the development of Salmonella BGs vaccines. Faculty of Food Science and Technology, Universiti Putra Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58375/1/%2862%29.pdf Thung, Tze Young and Chin, Yih Zhet and Mohd Shahril, Najwa and Anyi, Ubong and New, Chia Yeung and Bahumaish, Ramzi Othman Saeed and Kuan, Chee Hao and Mahyudin, Nor Ainy and Basri, Dayang Fredalina and Wan Mohamed Radzi, Che Wan Jasimah and Radu, Son (2017) Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat. International Food Research Journal, 24 (4). pp. 1826-1830. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/24%20(04)%202017/(62).pdf |
| spellingShingle | Thung, Tze Young Chin, Yih Zhet Mohd Shahril, Najwa Anyi, Ubong New, Chia Yeung Bahumaish, Ramzi Othman Saeed Kuan, Chee Hao Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title | Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title_full | Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title_fullStr | Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title_full_unstemmed | Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title_short | Construction of Salmonella Enteritidis and Salmonella Typhimurium ghosts isolated from retail chicken meat |
| title_sort | construction of salmonella enteritidis and salmonella typhimurium ghosts isolated from retail chicken meat |
| url | http://psasir.upm.edu.my/id/eprint/58375/ http://psasir.upm.edu.my/id/eprint/58375/ http://psasir.upm.edu.my/id/eprint/58375/1/%2862%29.pdf |