Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs)
The enzymatic synthesis of methyl adipate via green esterification of adipic acid and methanol in hexane has been studied. Lipase from Candida rugosa immobilised onto various layered double hydroxides (LDHs) by a reproducible and simple method of physical adsorption was used as biocatalyst with pro...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5822/ http://psasir.upm.edu.my/id/eprint/5822/1/Enzymatic%20synthesis%20of%20methyl%20adipate%20ester%20using%20lipase%20from%20Candida%20rugosa%20immobilised%20on%20Mg.pdf |
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| author | Abdul Rahman, Mohd Basyaruddin Zaidan, Uswatun Hasanah Basri, Mahiran Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_facet | Abdul Rahman, Mohd Basyaruddin Zaidan, Uswatun Hasanah Basri, Mahiran Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_sort | Abdul Rahman, Mohd Basyaruddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The enzymatic synthesis of methyl adipate via green esterification of adipic acid and methanol in hexane has been studied. Lipase from Candida rugosa immobilised onto various layered double hydroxides (LDHs) by a reproducible and simple method of physical adsorption was used as
biocatalyst with promising result. Mg/Al–NO3−, Zn/Al–NO3
− and Ni/Al–NO3− of LDHs with molar ratio of M2+/M3+ = 4:1 were synthesised by co-precipitation method with continuous agitation. The percentages of protein loading on Mg/Al–NO3
−, Zn/Al–NO − and Ni/Al–NO3 − were 71%, 67% and 58%, respectively, due to the larger surface area, porosity and basal spacing of the supports. Parameter studies of reaction time,reaction temperature, water activity, thermostability, storage, leaching and reusability were investigated and optimised. Optimum conditions to
produce adipate ester upto 80 % were reaction time; 2.5 h, temperature; 50 ◦C, and water activity; 0.53, respectively. Increased in optimisation conditions and enhanced stability properties were found after immobilisation compared to the native lipase. |
| first_indexed | 2025-11-15T07:23:39Z |
| format | Article |
| id | upm-5822 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:23:39Z |
| publishDate | 2008 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-58222016-09-28T01:44:38Z http://psasir.upm.edu.my/id/eprint/5822/ Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) Abdul Rahman, Mohd Basyaruddin Zaidan, Uswatun Hasanah Basri, Mahiran Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar The enzymatic synthesis of methyl adipate via green esterification of adipic acid and methanol in hexane has been studied. Lipase from Candida rugosa immobilised onto various layered double hydroxides (LDHs) by a reproducible and simple method of physical adsorption was used as biocatalyst with promising result. Mg/Al–NO3−, Zn/Al–NO3 − and Ni/Al–NO3− of LDHs with molar ratio of M2+/M3+ = 4:1 were synthesised by co-precipitation method with continuous agitation. The percentages of protein loading on Mg/Al–NO3 −, Zn/Al–NO − and Ni/Al–NO3 − were 71%, 67% and 58%, respectively, due to the larger surface area, porosity and basal spacing of the supports. Parameter studies of reaction time,reaction temperature, water activity, thermostability, storage, leaching and reusability were investigated and optimised. Optimum conditions to produce adipate ester upto 80 % were reaction time; 2.5 h, temperature; 50 ◦C, and water activity; 0.53, respectively. Increased in optimisation conditions and enhanced stability properties were found after immobilisation compared to the native lipase. Elsevier 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5822/1/Enzymatic%20synthesis%20of%20methyl%20adipate%20ester%20using%20lipase%20from%20Candida%20rugosa%20immobilised%20on%20Mg.pdf Abdul Rahman, Mohd Basyaruddin and Zaidan, Uswatun Hasanah and Basri, Mahiran and Hussein, Mohd Zobir and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2008) Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs). Journal of Molecular Catalysis B: Enzymatic, 50 (1). pp. 33-39. ISSN 1381-1177; ESSN: 1873-3158 http://www.sciencedirect.com/science/article/pii/S1381117707001907 10.1016/j.molcatb.2007.09.020 |
| spellingShingle | Abdul Rahman, Mohd Basyaruddin Zaidan, Uswatun Hasanah Basri, Mahiran Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title | Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title_full | Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title_fullStr | Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title_full_unstemmed | Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title_short | Enzymatic synthesis of methyl adipate ester using lipase from Candida rugosa immobilised on Mg, Zn and Ni of layered double hydroxides (LDHs) |
| title_sort | enzymatic synthesis of methyl adipate ester using lipase from candida rugosa immobilised on mg, zn and ni of layered double hydroxides (ldhs) |
| url | http://psasir.upm.edu.my/id/eprint/5822/ http://psasir.upm.edu.my/id/eprint/5822/ http://psasir.upm.edu.my/id/eprint/5822/ http://psasir.upm.edu.my/id/eprint/5822/1/Enzymatic%20synthesis%20of%20methyl%20adipate%20ester%20using%20lipase%20from%20Candida%20rugosa%20immobilised%20on%20Mg.pdf |