Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii
A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus...
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| Format: | Article |
| Language: | English English |
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Springer Wien
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5803/ http://psasir.upm.edu.my/id/eprint/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf |
| _version_ | 1848840201726590976 |
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| author | Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri |
| author_facet | Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri |
| author_sort | Loh, Hwei San |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus morphology was confirmed by electron microscopy. Its slow growth in cell culture indicated that the virus is belonging to subfamily Betaherpesvirinae. Electron microscopy techniques and immunohistochemistry confirmed the presence of herpesviral inclusion bodies and virus related particles in the cytoplasm and nucleus of infected cells. Hyperimmune serum against the Maastricht strain of RCMV revealed the virus identity in neutralization test, immunoperoxidase and immunofluorescence
techniques. Despite typical characteristics of CMV, the viral genome is significantly different from that of Maastricht, English, UPM/Sg and UPM/Kn strains.
The dissimilarities, which have not been reported before, had been confirmed by mean of restriction endonuclease analysis. The new RCMV strain, a virus that infects placenta and uterus of rats, has been named as ALL-03. |
| first_indexed | 2025-11-15T07:23:35Z |
| format | Article |
| id | upm-5803 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:23:35Z |
| publishDate | 2003 |
| publisher | Springer Wien |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-58032015-10-06T00:51:58Z http://psasir.upm.edu.my/id/eprint/5803/ Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus morphology was confirmed by electron microscopy. Its slow growth in cell culture indicated that the virus is belonging to subfamily Betaherpesvirinae. Electron microscopy techniques and immunohistochemistry confirmed the presence of herpesviral inclusion bodies and virus related particles in the cytoplasm and nucleus of infected cells. Hyperimmune serum against the Maastricht strain of RCMV revealed the virus identity in neutralization test, immunoperoxidase and immunofluorescence techniques. Despite typical characteristics of CMV, the viral genome is significantly different from that of Maastricht, English, UPM/Sg and UPM/Kn strains. The dissimilarities, which have not been reported before, had been confirmed by mean of restriction endonuclease analysis. The new RCMV strain, a virus that infects placenta and uterus of rats, has been named as ALL-03. Springer Wien 2003-08-18 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf Loh, Hwei San and Mohd Lila, Mohd Azmi and Lai, Kit Yee and Abdul Rahman, Sheikh Omar and Saad, Mohd Zamri (2003) Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii. Archives of Virology, 148 (12). pp. 2353-2367. ISSN 0304-8608 http://dx.doi.org/10.1007/s00705-003-0173-y 10.1007/s00705-003-0173-y English |
| spellingShingle | Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
| title | Characterization of a novel rat cytomegalovirus (RCMV)
infecting placenta-uterus of Rattus rattus diardii |
| title_full | Characterization of a novel rat cytomegalovirus (RCMV)
infecting placenta-uterus of Rattus rattus diardii |
| title_fullStr | Characterization of a novel rat cytomegalovirus (RCMV)
infecting placenta-uterus of Rattus rattus diardii |
| title_full_unstemmed | Characterization of a novel rat cytomegalovirus (RCMV)
infecting placenta-uterus of Rattus rattus diardii |
| title_short | Characterization of a novel rat cytomegalovirus (RCMV)
infecting placenta-uterus of Rattus rattus diardii |
| title_sort | characterization of a novel rat cytomegalovirus (rcmv)
infecting placenta-uterus of rattus rattus diardii |
| url | http://psasir.upm.edu.my/id/eprint/5803/ http://psasir.upm.edu.my/id/eprint/5803/ http://psasir.upm.edu.my/id/eprint/5803/ http://psasir.upm.edu.my/id/eprint/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf |