Comparison Of Extraction Methods For Detecting Hepatitis A Virus In Shellfish (Mystilus Galloprovincialis) Using Tissue Culture Titration, RT-Nested PCR And Real Time RT-PCR
Thirty commercial shellfish samples (22 cockles and 8 surf cockles) were collected from several locations around Serdang and tested for the presence of hepatitis A virus (HAV) using a modification of the glycine, polyethylene glycol, Tri-reagent, poly dT bead (GPTT) extraction protocol. None of the...
| Main Author: | Yong, Han Tek |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2009
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/5738/ http://psasir.upm.edu.my/id/eprint/5738/1/FSTM_2009_8_abstract.pdf |
Similar Items
Development of an Rt Nested PCR-Elisa Diagnostic Test for the Detection of Newcastle Disease Virus
by: Kho, Chiew Ling
Published: (1999)
by: Kho, Chiew Ling
Published: (1999)
Co-infections of hepatitis B patients with other hepatitis viruses and their serological, biological and prognostic markers
by: Hudu Abdullahi, Shuaibu
Published: (2017)
by: Hudu Abdullahi, Shuaibu
Published: (2017)
Performance of an RT-nested PCR ELISA for detection of Newcastle disease virus
by: Kho, C.L, et al.
Published: (2000)
by: Kho, C.L, et al.
Published: (2000)
Investigation of Hepatitis B virus entry mechanism
by: Raymond, Chidinma
Published: (2022)
by: Raymond, Chidinma
Published: (2022)
Novel entry pathways for Hepatitis B virus
by: Parmar, Devan
Published: (2023)
by: Parmar, Devan
Published: (2023)
Display of the pres regions of the surface antigen of Hepatitis B virus on M13 and T7 Bactetiophages
by: Kok, Wai Ling
Published: (2001)
by: Kok, Wai Ling
Published: (2001)
Molecular characterization of avian influenza virus isolate VRI1803/04 and development of real-time rt-pcr detection method
by: Zulkifli, Hazalina
Published: (2013)
by: Zulkifli, Hazalina
Published: (2013)
Important considerations in qRT-PCR
by: Phang, Ing Chia
Published: (2011)
by: Phang, Ing Chia
Published: (2011)
Use of reverse transcription-polymerase chain reaction (RT-PCR) for Cymbidium mosaic virus (CyMV) detection in orchids
by: Mat, Mazidah
Published: (2000)
by: Mat, Mazidah
Published: (2000)
Expression, purification and structural characterisation of Hepatitis B virus core antigen with an N-terminal extension
by: Yoon, Kam Yee
Published: (2013)
by: Yoon, Kam Yee
Published: (2013)
Detection of PLC-ΞΆ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
by: A. S., Fadzlina, et al.
Published: (2013)
by: A. S., Fadzlina, et al.
Published: (2013)
Development of anti-hepatitis B virus core antigen biosensor using gold nanoparticle-decorated reduced graphene oxide
by: Abd Muain, Mohamad Farid
Published: (2019)
by: Abd Muain, Mohamad Farid
Published: (2019)
Recombinant human L-ficolin directly neutralizes hepatitis C virus entry
by: Hamed, Mohamed R., et al.
Published: (2014)
by: Hamed, Mohamed R., et al.
Published: (2014)
Selection of High Affinity Peptides Against Hepatitis B Core Antigen from a Phage-Displayed Cyclic Peptide Library
by: Ho, Kok Lian
Published: (2002)
by: Ho, Kok Lian
Published: (2002)
Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.
by: Lee, Khai Wooi, et al.
Published: (2008)
by: Lee, Khai Wooi, et al.
Published: (2008)
Molecular Characterization of Malaysian Isolates of Newcastle Disease Viruses and the Development of a Duplex RT-PCR for Simultaneous Diagnosis of Newcastle Disease and Avian Influenza
by: Mekonnen, Ayalew Berhanu
Published: (2010)
by: Mekonnen, Ayalew Berhanu
Published: (2010)
Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
by: Saedi, Tayebeh Azam
Published: (2011)
by: Saedi, Tayebeh Azam
Published: (2011)
Development of taqman-based real-time rt-pcr assay for quantitative detection of feline morbillivirus (feMV) and phylogenetic analysis of Malaysian feMV isolates
by: Makhtar, Siti Tasnim
Published: (2021)
by: Makhtar, Siti Tasnim
Published: (2021)
Molecular characterization and methylation status of torque teno virus isolation among hepatitis patients in a public hospital in Pahang, Malaysia
by: Hazanuddin, Siti Nurzulaikha
Published: (2020)
by: Hazanuddin, Siti Nurzulaikha
Published: (2020)
Molecular epidemiology and antiviral resistance characterization of Hepatitis C virus circulating in Africa and Brazil
by: Adeboyejo, Kazeem Adeyinka
Published: (2020)
by: Adeboyejo, Kazeem Adeyinka
Published: (2020)
Targeted delivery of short hairpin RNA expressing plasmid using hepatitis B virus-like particle for bcl-2 gene silencing in cervical cancer cells
by: Akwiditya, Made Angga
Published: (2021)
by: Akwiditya, Made Angga
Published: (2021)
Shielding of virus-like nanoparticles of hepatitis B core antigen by poly(2-oxazoline) for reduced antigenicity
by: Fam, See Yee
Published: (2021)
by: Fam, See Yee
Published: (2021)
Hepatitis B capsid as a delivery vehicle into mammalian cells
by: Lee, Khai Wooi
Published: (2012)
by: Lee, Khai Wooi
Published: (2012)
Splitting the eggs methods: Comparison of egg survivorship between styrofoam and open area nests for Penang Island turtle conservation
by: Mohd Salleh, Sarahaizad, et al.
Published: (2017)
by: Mohd Salleh, Sarahaizad, et al.
Published: (2017)
Development and evaluation of real time RT-PCR assays for detection and typing of Bluetongue virus
by: Richard, Yolande, et al.
Published: (2016)
by: Richard, Yolande, et al.
Published: (2016)
Differentiation of mass-serotype in infectious bronchitis virus local isolates using RT-PCR
by: A. Talip, Balkis, et al.
Published: (2004)
by: A. Talip, Balkis, et al.
Published: (2004)
Hepatic elastin content is predictive of adverse outcome in advanced fibrotic liver disease
by: Kendall, Timothy, et al.
Published: (2018)
by: Kendall, Timothy, et al.
Published: (2018)
Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7
by: Tan, Geok Hun, et al.
Published: (2005)
by: Tan, Geok Hun, et al.
Published: (2005)
Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
by: Yee, May Ye
Published: (2020)
by: Yee, May Ye
Published: (2020)
Targeted 5-fluorouracil-1-acetic acid delivery utilising cell penetrating peptide conjugated hepatitis b virus-like nanoparticles to squamous cell carcinoma A431
by: Gan, Bee Koon
Published: (2020)
by: Gan, Bee Koon
Published: (2020)
Safety issues related to polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in fish and shellfish in relation with current Malaysian laws.
by: Azlan, Azrina, et al.
Published: (2009)
by: Azlan, Azrina, et al.
Published: (2009)
Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance
by: Lareu, Ricky R., et al.
Published: (2007)
by: Lareu, Ricky R., et al.
Published: (2007)
Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae
by: Abdullah Al-Haj, Nagi Ahmed, et al.
Published: (2009)
by: Abdullah Al-Haj, Nagi Ahmed, et al.
Published: (2009)
Detection of citrus viroids by multiplex reverse transcription polymerase chain reaction (RT-PCR)
by: Roslin, Nor Athirah
Published: (2017)
by: Roslin, Nor Athirah
Published: (2017)
Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm
by: Roslan, Nur Diyana, et al.
Published: (2023)
by: Roslan, Nur Diyana, et al.
Published: (2023)
The effects of the human innate pattern recognition receptor L-ficolin and its variants against Hepatitis C virus infection
by: Mason, Christopher Paul
Published: (2019)
by: Mason, Christopher Paul
Published: (2019)
Purification and characterization of polyclonal anti-hepatitis B core antigen immunoglobulin G from rabbit serum
by: Sayed Hitam, Sharifah Mariam
Published: (2016)
by: Sayed Hitam, Sharifah Mariam
Published: (2016)
Authenticity and Diversity: Enhancing Australian Hepatitis C Prevention Messages
by: Winter, R., et al.
Published: (2013)
by: Winter, R., et al.
Published: (2013)
Molecular prevalence and genome analysis of domestic cat hepadnavirus in Malaysia
by: Anpuanandam, Khanmani
Published: (2021)
by: Anpuanandam, Khanmani
Published: (2021)
Selection of Stable Housekeeping Gene for Quantitative Real-time PCR (qRT-CR) Analysis in Human Normal and Cancerous Tissues
by: Lasim, Masniza Muhamad
Published: (2014)
by: Lasim, Masniza Muhamad
Published: (2014)
Similar Items
-
Development of an Rt Nested PCR-Elisa Diagnostic Test for the Detection of Newcastle Disease Virus
by: Kho, Chiew Ling
Published: (1999) -
Co-infections of hepatitis B patients with other hepatitis viruses and their serological, biological and prognostic markers
by: Hudu Abdullahi, Shuaibu
Published: (2017) -
Performance of an RT-nested PCR ELISA for detection of Newcastle disease virus
by: Kho, C.L, et al.
Published: (2000) -
Investigation of Hepatitis B virus entry mechanism
by: Raymond, Chidinma
Published: (2022) -
Novel entry pathways for Hepatitis B virus
by: Parmar, Devan
Published: (2023)