Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats
Objective: Black tea and Nonileaf are among the dietary compounds that can benefit patients with bone resorption disorders. Their bone regeneration effects and their mechanisms were studied in estrogen-deficient rats. Methods: Noni leaves (three doses) and black tea water extracts were fed to ovari...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56977/ http://psasir.upm.edu.my/id/eprint/56977/1/Noni%20leaf%20and%20black%20tea%20enhance%20bone%20regeneration%20in%20estrogen-deficient%20rats.pdf |
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| author | Mohd Shalan, Nor Aijratul Asikin M. Mustapha, Noordin Mohamed, Suhaila |
| author_facet | Mohd Shalan, Nor Aijratul Asikin M. Mustapha, Noordin Mohamed, Suhaila |
| author_sort | Mohd Shalan, Nor Aijratul Asikin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: Black tea and Nonileaf are among the dietary compounds that can benefit patients with bone resorption disorders. Their bone regeneration effects and their mechanisms were studied in estrogen-deficient rats.
Methods: Noni leaves (three doses) and black tea water extracts were fed to ovariectomized rats for 4 mo, and their effects (analyzed via mechanical measurements, micro-computed tomography scan, and reverse transcriptase polymerase chain reaction mRNA) were compared with Remifemin (a commercial phytoestrogen product from black cohosh).
Results: The water extracts (dose-dependently for noni leaves) increased bone regeneration biomarker (runt-related transcription factor 2, bone morphogenetic protein 2, osteoprotegerin, estrogen receptor 1 [ESR1], collagen type I alpha 1A) expressions and reduced the inflammatory biomarkers (interleukin-6, tumor necrosis factor-α, nuclear factor [NF]-κB, and receptor activator of NF-κB ligand) mRNA expressions/levels in the rats. The extracts also improved bone physical and mechanical properties. The extracts demonstrated bone regeneration through improving bone size and structure, bone mechanical properties (strength and flexibility), and bone mineralization and density.
Conclusions: The catechin-rich extract favored bone regeneration and suppressed bone resorption. The mechanisms involved enhancing osteoblast generation and survival, inhibiting osteoclast growth and activities, suppressing inflammation, improving bone collagen synthesis and upregulating ESR1 expression to augment phytoestrogenic effects. Estrogen deficiency bone loss and all extracts studied (best effect from Morinda leaf at 300 mg/kg body weight) mitigated the loss, indicating benefits for the aged and menopausal women. |
| first_indexed | 2025-11-15T10:50:47Z |
| format | Article |
| id | upm-56977 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:50:47Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-569772017-09-07T03:09:27Z http://psasir.upm.edu.my/id/eprint/56977/ Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats Mohd Shalan, Nor Aijratul Asikin M. Mustapha, Noordin Mohamed, Suhaila Objective: Black tea and Nonileaf are among the dietary compounds that can benefit patients with bone resorption disorders. Their bone regeneration effects and their mechanisms were studied in estrogen-deficient rats. Methods: Noni leaves (three doses) and black tea water extracts were fed to ovariectomized rats for 4 mo, and their effects (analyzed via mechanical measurements, micro-computed tomography scan, and reverse transcriptase polymerase chain reaction mRNA) were compared with Remifemin (a commercial phytoestrogen product from black cohosh). Results: The water extracts (dose-dependently for noni leaves) increased bone regeneration biomarker (runt-related transcription factor 2, bone morphogenetic protein 2, osteoprotegerin, estrogen receptor 1 [ESR1], collagen type I alpha 1A) expressions and reduced the inflammatory biomarkers (interleukin-6, tumor necrosis factor-α, nuclear factor [NF]-κB, and receptor activator of NF-κB ligand) mRNA expressions/levels in the rats. The extracts also improved bone physical and mechanical properties. The extracts demonstrated bone regeneration through improving bone size and structure, bone mechanical properties (strength and flexibility), and bone mineralization and density. Conclusions: The catechin-rich extract favored bone regeneration and suppressed bone resorption. The mechanisms involved enhancing osteoblast generation and survival, inhibiting osteoclast growth and activities, suppressing inflammation, improving bone collagen synthesis and upregulating ESR1 expression to augment phytoestrogenic effects. Estrogen deficiency bone loss and all extracts studied (best effect from Morinda leaf at 300 mg/kg body weight) mitigated the loss, indicating benefits for the aged and menopausal women. Elsevier 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56977/1/Noni%20leaf%20and%20black%20tea%20enhance%20bone%20regeneration%20in%20estrogen-deficient%20rats.pdf Mohd Shalan, Nor Aijratul Asikin and M. Mustapha, Noordin and Mohamed, Suhaila (2017) Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. Nutrition, 33. pp. 42-51. ISSN 0899-9007; ESSN: 1873-1244 http://www.sciencedirect.com/science/article/pii/S0899900716301976?via%3Dihub#! 10.1016/j.nut.2016.08.006 |
| spellingShingle | Mohd Shalan, Nor Aijratul Asikin M. Mustapha, Noordin Mohamed, Suhaila Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title_full | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title_fullStr | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title_full_unstemmed | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title_short | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| title_sort | noni leaf and black tea enhance bone regeneration in estrogen-deficient rats |
| url | http://psasir.upm.edu.my/id/eprint/56977/ http://psasir.upm.edu.my/id/eprint/56977/ http://psasir.upm.edu.my/id/eprint/56977/ http://psasir.upm.edu.my/id/eprint/56977/1/Noni%20leaf%20and%20black%20tea%20enhance%20bone%20regeneration%20in%20estrogen-deficient%20rats.pdf |