Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method
Mesenchymal stem cells (MSCs) derived from human umbilical cord (UC) have been considered as an important tool for treating various malignancies, tissue repair and organ regeneration. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are better alternative to MSCs that derived from bone marrow...
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| Format: | Article |
| Language: | English |
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Malaysian Medical Association
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56862/ http://psasir.upm.edu.my/id/eprint/56862/1/Generation%20and%20characterisation%20of%20human%20umbilical%20cord%20derived%20mesenchymal%20stem%20cells%20by%20explant%20method.pdf |
| _version_ | 1848853204449624064 |
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| author | Yusoff, Zuraidah Maqbool, Maryam George, Elizabeth Hassan, Roshida Ramasamy, Rajesh |
| author_facet | Yusoff, Zuraidah Maqbool, Maryam George, Elizabeth Hassan, Roshida Ramasamy, Rajesh |
| author_sort | Yusoff, Zuraidah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mesenchymal stem cells (MSCs) derived from human umbilical cord (UC) have been considered as an important tool for treating various malignancies, tissue repair and organ regeneration. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are better alternative to MSCs that derived from bone marrow (BM-MSCs) as they are regarded as medical waste with little ethical concern for research and easily culture-expanded. In this present study, the foetal distal end of human UC was utilised to generate MSC by explant method. Upon in vitro culture, adherent cells with fibroblastic morphology were generated with rapid growth kinetics. Under the respective inductive conditions, these cells were capable of differentiating into adipocytes and osteocytes; express an array of standard MSC’s surface markers CD29, CD73, CD90, CD106 and MHC-class I. Further assessment of immunosuppression activity revealed that MSCs generated from UC had profoundly inhibited the proliferation of mitogen-activated T lymphocytes in a dosedependent manner. The current laboratory findings have reinforced the application of explant method to generate UCMSCs thus, exploring an ideal platform to fulfil the increasing demand of MSCs for research and potential clinical use. |
| first_indexed | 2025-11-15T10:50:15Z |
| format | Article |
| id | upm-56862 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:50:15Z |
| publishDate | 2016 |
| publisher | Malaysian Medical Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-568622017-09-05T10:05:42Z http://psasir.upm.edu.my/id/eprint/56862/ Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method Yusoff, Zuraidah Maqbool, Maryam George, Elizabeth Hassan, Roshida Ramasamy, Rajesh Mesenchymal stem cells (MSCs) derived from human umbilical cord (UC) have been considered as an important tool for treating various malignancies, tissue repair and organ regeneration. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are better alternative to MSCs that derived from bone marrow (BM-MSCs) as they are regarded as medical waste with little ethical concern for research and easily culture-expanded. In this present study, the foetal distal end of human UC was utilised to generate MSC by explant method. Upon in vitro culture, adherent cells with fibroblastic morphology were generated with rapid growth kinetics. Under the respective inductive conditions, these cells were capable of differentiating into adipocytes and osteocytes; express an array of standard MSC’s surface markers CD29, CD73, CD90, CD106 and MHC-class I. Further assessment of immunosuppression activity revealed that MSCs generated from UC had profoundly inhibited the proliferation of mitogen-activated T lymphocytes in a dosedependent manner. The current laboratory findings have reinforced the application of explant method to generate UCMSCs thus, exploring an ideal platform to fulfil the increasing demand of MSCs for research and potential clinical use. Malaysian Medical Association 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56862/1/Generation%20and%20characterisation%20of%20human%20umbilical%20cord%20derived%20mesenchymal%20stem%20cells%20by%20explant%20method.pdf Yusoff, Zuraidah and Maqbool, Maryam and George, Elizabeth and Hassan, Roshida and Ramasamy, Rajesh (2016) Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method. Medical Journal of Malaysia, 71 (3). pp. 105-110. ISSN 0300-5283 http://www.e-mjm.org/2016/v71n3/index.html |
| spellingShingle | Yusoff, Zuraidah Maqbool, Maryam George, Elizabeth Hassan, Roshida Ramasamy, Rajesh Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title | Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title_full | Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title_fullStr | Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title_full_unstemmed | Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title_short | Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| title_sort | generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method |
| url | http://psasir.upm.edu.my/id/eprint/56862/ http://psasir.upm.edu.my/id/eprint/56862/ http://psasir.upm.edu.my/id/eprint/56862/1/Generation%20and%20characterisation%20of%20human%20umbilical%20cord%20derived%20mesenchymal%20stem%20cells%20by%20explant%20method.pdf |