Cytotoxic activity of coumarins from Micromelum minutum
The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihyd...
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| Format: | Article |
| Language: | English |
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Informa Healthcare
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56238/ http://psasir.upm.edu.my/id/eprint/56238/1/Cytotoxic%20activity%20of%20coumarins%20from%20Micromelum%20minutum.pdf |
| _version_ | 1848853026403516416 |
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| author | Susidarti, Ratna Asmah Rahmani, Mawardi Mohd Ismail, Hazar Bebe Md. Sukari, Mohd Aspollah Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Ali, Abdul Manaf |
| author_facet | Susidarti, Ratna Asmah Rahmani, Mawardi Mohd Ismail, Hazar Bebe Md. Sukari, Mohd Aspollah Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Ali, Abdul Manaf |
| author_sort | Susidarti, Ratna Asmah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihydrocapnolactone, 2′,3′-epoxyisocapnolactone, 8-hydroxyisocapnolactone-29,39-diol, 8-hydroxy-3″,4″- dihydrocapnolactone-29,39-diol and 8,4″-dihydroxy-3″,4″- dihydrocapnolactone-29,39-diol, and two triterpenes. Some of these compounds were strongly active against T-lymphoblastic leukemia (CEM-SS), promyeolocytic leukemia (HL60), cervical cancer (HeLa) and liver cancer (HepG2) cell lines. 8-Hydroxyisocapnolactone-29,39-diol was found to be the most active with IC50 values of 2.9, 2.5, 6.9, and 5.9 μg/ml, respectively. This was followed by 2′,3′-epoxyisocapnolactone. When evaluated against the normal mouse fibroblast (3T3) cell line, 8-hydroxyisocapnolactone-29,39-diol was found to be inactive, hence it could serve as a valuable lead for further design and synthesis of more active analogues. |
| first_indexed | 2025-11-15T10:47:26Z |
| format | Article |
| id | upm-56238 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:47:26Z |
| publishDate | 2009 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-562382017-07-04T02:48:06Z http://psasir.upm.edu.my/id/eprint/56238/ Cytotoxic activity of coumarins from Micromelum minutum Susidarti, Ratna Asmah Rahmani, Mawardi Mohd Ismail, Hazar Bebe Md. Sukari, Mohd Aspollah Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Ali, Abdul Manaf The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihydrocapnolactone, 2′,3′-epoxyisocapnolactone, 8-hydroxyisocapnolactone-29,39-diol, 8-hydroxy-3″,4″- dihydrocapnolactone-29,39-diol and 8,4″-dihydroxy-3″,4″- dihydrocapnolactone-29,39-diol, and two triterpenes. Some of these compounds were strongly active against T-lymphoblastic leukemia (CEM-SS), promyeolocytic leukemia (HL60), cervical cancer (HeLa) and liver cancer (HepG2) cell lines. 8-Hydroxyisocapnolactone-29,39-diol was found to be the most active with IC50 values of 2.9, 2.5, 6.9, and 5.9 μg/ml, respectively. This was followed by 2′,3′-epoxyisocapnolactone. When evaluated against the normal mouse fibroblast (3T3) cell line, 8-hydroxyisocapnolactone-29,39-diol was found to be inactive, hence it could serve as a valuable lead for further design and synthesis of more active analogues. Informa Healthcare 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56238/1/Cytotoxic%20activity%20of%20coumarins%20from%20Micromelum%20minutum.pdf Susidarti, Ratna Asmah and Rahmani, Mawardi and Mohd Ismail, Hazar Bebe and Md. Sukari, Mohd Aspollah and Yap, Taufiq Yun Hin and Ee, Gwendoline Cheng Lian and Ali, Abdul Manaf (2009) Cytotoxic activity of coumarins from Micromelum minutum. Pharmaceutical Biology, 47 (2). pp. 182-185. ISSN 1388-0209; ESSN: 1744-5116 http://www.tandfonline.com/doi/abs/10.1080/13880200802436513 10.1080/13880200802436513 |
| spellingShingle | Susidarti, Ratna Asmah Rahmani, Mawardi Mohd Ismail, Hazar Bebe Md. Sukari, Mohd Aspollah Yap, Taufiq Yun Hin Ee, Gwendoline Cheng Lian Ali, Abdul Manaf Cytotoxic activity of coumarins from Micromelum minutum |
| title | Cytotoxic activity of coumarins from Micromelum minutum |
| title_full | Cytotoxic activity of coumarins from Micromelum minutum |
| title_fullStr | Cytotoxic activity of coumarins from Micromelum minutum |
| title_full_unstemmed | Cytotoxic activity of coumarins from Micromelum minutum |
| title_short | Cytotoxic activity of coumarins from Micromelum minutum |
| title_sort | cytotoxic activity of coumarins from micromelum minutum |
| url | http://psasir.upm.edu.my/id/eprint/56238/ http://psasir.upm.edu.my/id/eprint/56238/ http://psasir.upm.edu.my/id/eprint/56238/ http://psasir.upm.edu.my/id/eprint/56238/1/Cytotoxic%20activity%20of%20coumarins%20from%20Micromelum%20minutum.pdf |