The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed
Ten solvents were used to extract phytochemicals from the peel of Carica papaya cv. Sekaki/Hong Kong to evaluate antibacterial activities and determine chemical composition of Carica papaya cv. Sekaki/Hong Kong seeds. The antibacterial activities of ten solvent extracts were tested against 14 microo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/56141/ http://psasir.upm.edu.my/id/eprint/56141/1/%2848%29.pdf |
| _version_ | 1848852999037779968 |
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| author | Muhamad, S. A. S. Bakar, Jamilah Abdul Rahman, Russly A., Faridah |
| author_facet | Muhamad, S. A. S. Bakar, Jamilah Abdul Rahman, Russly A., Faridah |
| author_sort | Muhamad, S. A. S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Ten solvents were used to extract phytochemicals from the peel of Carica papaya cv. Sekaki/Hong Kong to evaluate antibacterial activities and determine chemical composition of Carica papaya cv. Sekaki/Hong Kong seeds. The antibacterial activities of ten solvent extracts were tested against 14 microorganisms vis Shigella sonnei, Salmonella typhimurium, Escherichia coli, Salmonella enteritidis, Vibrio vulnificus, Vibrio parahaemolyticus, Proteus mirabilis, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Corynebacterium diphtheria, Clostridium perfringens, Streptococcus pneumoniae and Bacillus subtilis using disk diffusion test (DDT). The
Carica papaya seed of ACN and MeOH extracts inhibited 11 microorganisms, indicating their broad spectrum activity. The disk diffusion test exhibited moderate and clear inhibition on C. diphtheria, S. pneumonia, B. subtilis and C. perfringens. MeOH extract inhibited S. enteritidis, V. vulnificus, P. mirabilis and B. cereus with the lowest MIC at 11.25 mg/mL each, thus was chosen as the best extract. The hierarchy of extract potency can be ranked as MeOH > acetone > ACN > CHCL3 > hexane > DE = PE > EtOH > DCM based on clear and moderate inhibition and the lowest MIC. TPC and TFC of the extracts ranged between 4.83 to 22.59 mg GAE/g DW and 1.32 (water) and 17.15 mg QE/g DW respectively. The GC/MS analysis of MeOH extract identified potential antibacterial compounds such as isothiocyanatomethyl benzene, 9-octadecenoic acid, hexadecanoic acid and β-sitosterol. The Carica papaya seed cv. Sekaki/ Hong Kong possessed significant antibacterial activities when extracted by different solvents in particular MeOH solvent. |
| first_indexed | 2025-11-15T10:46:59Z |
| format | Article |
| id | upm-56141 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:46:59Z |
| publishDate | 2017 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-561412017-07-05T03:20:25Z http://psasir.upm.edu.my/id/eprint/56141/ The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed Muhamad, S. A. S. Bakar, Jamilah Abdul Rahman, Russly A., Faridah Ten solvents were used to extract phytochemicals from the peel of Carica papaya cv. Sekaki/Hong Kong to evaluate antibacterial activities and determine chemical composition of Carica papaya cv. Sekaki/Hong Kong seeds. The antibacterial activities of ten solvent extracts were tested against 14 microorganisms vis Shigella sonnei, Salmonella typhimurium, Escherichia coli, Salmonella enteritidis, Vibrio vulnificus, Vibrio parahaemolyticus, Proteus mirabilis, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Corynebacterium diphtheria, Clostridium perfringens, Streptococcus pneumoniae and Bacillus subtilis using disk diffusion test (DDT). The Carica papaya seed of ACN and MeOH extracts inhibited 11 microorganisms, indicating their broad spectrum activity. The disk diffusion test exhibited moderate and clear inhibition on C. diphtheria, S. pneumonia, B. subtilis and C. perfringens. MeOH extract inhibited S. enteritidis, V. vulnificus, P. mirabilis and B. cereus with the lowest MIC at 11.25 mg/mL each, thus was chosen as the best extract. The hierarchy of extract potency can be ranked as MeOH > acetone > ACN > CHCL3 > hexane > DE = PE > EtOH > DCM based on clear and moderate inhibition and the lowest MIC. TPC and TFC of the extracts ranged between 4.83 to 22.59 mg GAE/g DW and 1.32 (water) and 17.15 mg QE/g DW respectively. The GC/MS analysis of MeOH extract identified potential antibacterial compounds such as isothiocyanatomethyl benzene, 9-octadecenoic acid, hexadecanoic acid and β-sitosterol. The Carica papaya seed cv. Sekaki/ Hong Kong possessed significant antibacterial activities when extracted by different solvents in particular MeOH solvent. Faculty of Food Science and Technology, Universiti Putra Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/56141/1/%2848%29.pdf Muhamad, S. A. S. and Bakar, Jamilah and Abdul Rahman, Russly and A., Faridah (2017) The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed. International Food Research Journal, 24 (2). pp. 810-818. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/24%20(02)%202017/(48).pdf |
| spellingShingle | Muhamad, S. A. S. Bakar, Jamilah Abdul Rahman, Russly A., Faridah The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title | The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title_full | The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title_fullStr | The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title_full_unstemmed | The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title_short | The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed |
| title_sort | antibacterial activities and chemical composition of extracts from carica papaya cv. sekaki/hong kong seed |
| url | http://psasir.upm.edu.my/id/eprint/56141/ http://psasir.upm.edu.my/id/eprint/56141/ http://psasir.upm.edu.my/id/eprint/56141/1/%2848%29.pdf |