Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators
Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp. italica) cv. Green Marvel. The explants were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog (MS) and vari...
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/5591/ http://psasir.upm.edu.my/id/eprint/5591/1/Plant_regeneration_of_Brassica_oleracea.pdf |
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| author | Ravanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Sirchi, Mohammad Hossein Torabi |
| author_facet | Ravanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Sirchi, Mohammad Hossein Torabi |
| author_sort | Ravanfar, Seyed Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp. italica) cv. Green Marvel. The explants were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). The highest percentage of hypocotyl explant producing shoot (96.67%) and the highest mean number of shoots produced per hypocotyl explant (6.03) were obtained on 3 mgL-1BAP. Meanwhile, the highest percentage of shoot tip explant producing shoot (100%) and highest number of shoot produced per shoot tip explant (3.76) were recorded on 5 mgL-1 BAP. For rooting of shoots, NAA, indoleacetic acid (IAA) and indolebutyric acid (IBA) at 0, 0.2 and 1 mgL-1 were applied. Highest percentage of shoots with roots (100%) and highest mean number of roots produced per shoot (6.5) occurred on medium with 0.2 mgL-1 IBA, while the maximum root length (2.46 cm) was attained on MS medium without plant growth regulator (MSO). Plantlets were successfully acclimatized in potting medium containing peatmoss, perlite, and vermiculite (3:1:1). |
| first_indexed | 2025-11-15T07:22:38Z |
| format | Article |
| id | upm-5591 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:22:38Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-55912017-11-14T02:08:15Z http://psasir.upm.edu.my/id/eprint/5591/ Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators Ravanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Sirchi, Mohammad Hossein Torabi Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp. italica) cv. Green Marvel. The explants were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). The highest percentage of hypocotyl explant producing shoot (96.67%) and the highest mean number of shoots produced per hypocotyl explant (6.03) were obtained on 3 mgL-1BAP. Meanwhile, the highest percentage of shoot tip explant producing shoot (100%) and highest number of shoot produced per shoot tip explant (3.76) were recorded on 5 mgL-1 BAP. For rooting of shoots, NAA, indoleacetic acid (IAA) and indolebutyric acid (IBA) at 0, 0.2 and 1 mgL-1 were applied. Highest percentage of shoots with roots (100%) and highest mean number of roots produced per shoot (6.5) occurred on medium with 0.2 mgL-1 IBA, while the maximum root length (2.46 cm) was attained on MS medium without plant growth regulator (MSO). Plantlets were successfully acclimatized in potting medium containing peatmoss, perlite, and vermiculite (3:1:1). Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/5591/1/Plant_regeneration_of_Brassica_oleracea.pdf Ravanfar, Seyed Ali and Abdul Aziz, Maheran and Abdul Kadir, Mihdzar and Abdul Rashid, Azmi and Sirchi, Mohammad Hossein Torabi (2009) Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators. African Journal of Biotechnology, 8 (11). art. no. 92D2A277073. pp. 2523-2528. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/92D2A277073 |
| spellingShingle | Ravanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Sirchi, Mohammad Hossein Torabi Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title | Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title_full | Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title_fullStr | Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title_full_unstemmed | Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title_short | Plant regeneration of Brassica oleracea sub sp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators |
| title_sort | plant regeneration of brassica oleracea sub sp. italica (broccoli) cv green marvel as affected by plant growth regulators |
| url | http://psasir.upm.edu.my/id/eprint/5591/ http://psasir.upm.edu.my/id/eprint/5591/ http://psasir.upm.edu.my/id/eprint/5591/1/Plant_regeneration_of_Brassica_oleracea.pdf |