Crude oil biodegradation using isolated bacteria from oil polluted soil

Contamination due to the spillage of petroleum or petroleum-derived hydrocarbons on different ecosystems has become critical issues to environment and living things. The use of native bacteria as an environmental friendly treatment of hydrocarbons pollution is very promising. The objective of the st...

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Main Authors: Alavijeh, Pardis Karimi, Halimoon, Normala, Zakaria, Mohamad Pauzi, Wan Johari, Wan Lutfi, Karimi, Benyamin
Format: Conference or Workshop Item
Language:English
Published: Springer 2013
Online Access:http://psasir.upm.edu.my/id/eprint/55725/
http://psasir.upm.edu.my/id/eprint/55725/1/Crude%20oil%20biodegradation%20using%20isolated%20bacteria%20from%20oil%20polluted%20soil.pdf
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author Alavijeh, Pardis Karimi
Halimoon, Normala
Zakaria, Mohamad Pauzi
Wan Johari, Wan Lutfi
Karimi, Benyamin
author_facet Alavijeh, Pardis Karimi
Halimoon, Normala
Zakaria, Mohamad Pauzi
Wan Johari, Wan Lutfi
Karimi, Benyamin
author_sort Alavijeh, Pardis Karimi
building UPM Institutional Repository
collection Online Access
description Contamination due to the spillage of petroleum or petroleum-derived hydrocarbons on different ecosystems has become critical issues to environment and living things. The use of native bacteria as an environmental friendly treatment of hydrocarbons pollution is very promising. The objective of the study is to identify the native hydrocarbon-degraders isolated from oil refinery. Bacteria from crude oil were isolated and cultured in enrichment media. The isolated strains were then cultured in different oil concentration. The isolated bacterial strains were Gram-stained and further identified via 16S rRNA sequencing. The resultant sequences were then aligned with the available online database for identification of the bacteria. The isolated bacteria belonged to families of Pseudomonadaceae and Moraxellaceae (Acinetobacter genus). The bacteria show high degradation of hydrocarbon and can be used to remediate polluted soils in tropical environment.
first_indexed 2025-11-15T10:45:08Z
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T10:45:08Z
publishDate 2013
publisher Springer
recordtype eprints
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spelling upm-557252017-06-07T08:44:10Z http://psasir.upm.edu.my/id/eprint/55725/ Crude oil biodegradation using isolated bacteria from oil polluted soil Alavijeh, Pardis Karimi Halimoon, Normala Zakaria, Mohamad Pauzi Wan Johari, Wan Lutfi Karimi, Benyamin Contamination due to the spillage of petroleum or petroleum-derived hydrocarbons on different ecosystems has become critical issues to environment and living things. The use of native bacteria as an environmental friendly treatment of hydrocarbons pollution is very promising. The objective of the study is to identify the native hydrocarbon-degraders isolated from oil refinery. Bacteria from crude oil were isolated and cultured in enrichment media. The isolated strains were then cultured in different oil concentration. The isolated bacterial strains were Gram-stained and further identified via 16S rRNA sequencing. The resultant sequences were then aligned with the available online database for identification of the bacteria. The isolated bacteria belonged to families of Pseudomonadaceae and Moraxellaceae (Acinetobacter genus). The bacteria show high degradation of hydrocarbon and can be used to remediate polluted soils in tropical environment. Springer 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55725/1/Crude%20oil%20biodegradation%20using%20isolated%20bacteria%20from%20oil%20polluted%20soil.pdf Alavijeh, Pardis Karimi and Halimoon, Normala and Zakaria, Mohamad Pauzi and Wan Johari, Wan Lutfi and Karimi, Benyamin (2013) Crude oil biodegradation using isolated bacteria from oil polluted soil. In: International Conference on Environmental Forensics 2013, 11-14 Nov. 2013, Marriot Hotel, Putrajaya, Malaysia. (pp. 127-129). 10.1007/978-981-4560-70-2_24
spellingShingle Alavijeh, Pardis Karimi
Halimoon, Normala
Zakaria, Mohamad Pauzi
Wan Johari, Wan Lutfi
Karimi, Benyamin
Crude oil biodegradation using isolated bacteria from oil polluted soil
title Crude oil biodegradation using isolated bacteria from oil polluted soil
title_full Crude oil biodegradation using isolated bacteria from oil polluted soil
title_fullStr Crude oil biodegradation using isolated bacteria from oil polluted soil
title_full_unstemmed Crude oil biodegradation using isolated bacteria from oil polluted soil
title_short Crude oil biodegradation using isolated bacteria from oil polluted soil
title_sort crude oil biodegradation using isolated bacteria from oil polluted soil
url http://psasir.upm.edu.my/id/eprint/55725/
http://psasir.upm.edu.my/id/eprint/55725/
http://psasir.upm.edu.my/id/eprint/55725/1/Crude%20oil%20biodegradation%20using%20isolated%20bacteria%20from%20oil%20polluted%20soil.pdf