Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients
Background: Candida albicans (C. albicans) has several virulence factors, in particular heat shock protein 90 (Hsp90), which is expressed by Hsp90 gene. The purposes of this study were to assess the expression of Hsp90 gene in clinical and control isolates of C. albicans obtained from different geog...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55525/ http://psasir.upm.edu.my/id/eprint/55525/1/Differential%20gene%20expression%20of%20heat%20shock%20protein%2090%20%28Hsp90%29%20of%20Candida%20Albicans%20.pdf |
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| author | Khalili, Vajihe Shokri, Hojjatollah Md Akim, Abdah Khosravi, Ali Reza |
| author_facet | Khalili, Vajihe Shokri, Hojjatollah Md Akim, Abdah Khosravi, Ali Reza |
| author_sort | Khalili, Vajihe |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Candida albicans (C. albicans) has several virulence factors, in particular heat shock protein 90 (Hsp90), which is expressed by Hsp90 gene. The purposes of this study were to assess the expression of Hsp90 gene in clinical and control isolates of C. albicans obtained from different geographical regions (Malaysia and Iran), different temperatures (25°C, 37°C and 42°C) and mice with candidiasis.
Methods: C. albicans isolates were cultured onto sabouraud dextrose agar (SDA). The assessment of the expression of Hsp90 gene was performed using real time-polymerase chain reaction (RT-PCR).
Results: The results showed a significant increase in the expression of C. albicans Hsp90 gene under high thermal shock (42°C) when compared to other temperatures tested (P-value = 0.001). The mean differences in the expression of Hsp90 gene at 37°C were 0.20 (95% confidence interval (CI) 0.13-0.29) between Malaysian and Iranian controls (P-value = 0.040) and 0.47 (95% CI 0.27-0.60) between Malaysian and Iranian patients (P-value = 0.040).
Conclusion: The results demonstrated that the expression of C. albicans Hsp90 gene varied between Malaysian and Iranian subjects, representing the efficacy of geographical and thermal conditions on virulence gene expression. |
| first_indexed | 2025-11-15T10:44:15Z |
| format | Article |
| id | upm-55525 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:44:15Z |
| publishDate | 2016 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-555252017-09-13T02:31:44Z http://psasir.upm.edu.my/id/eprint/55525/ Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients Khalili, Vajihe Shokri, Hojjatollah Md Akim, Abdah Khosravi, Ali Reza Background: Candida albicans (C. albicans) has several virulence factors, in particular heat shock protein 90 (Hsp90), which is expressed by Hsp90 gene. The purposes of this study were to assess the expression of Hsp90 gene in clinical and control isolates of C. albicans obtained from different geographical regions (Malaysia and Iran), different temperatures (25°C, 37°C and 42°C) and mice with candidiasis. Methods: C. albicans isolates were cultured onto sabouraud dextrose agar (SDA). The assessment of the expression of Hsp90 gene was performed using real time-polymerase chain reaction (RT-PCR). Results: The results showed a significant increase in the expression of C. albicans Hsp90 gene under high thermal shock (42°C) when compared to other temperatures tested (P-value = 0.001). The mean differences in the expression of Hsp90 gene at 37°C were 0.20 (95% confidence interval (CI) 0.13-0.29) between Malaysian and Iranian controls (P-value = 0.040) and 0.47 (95% CI 0.27-0.60) between Malaysian and Iranian patients (P-value = 0.040). Conclusion: The results demonstrated that the expression of C. albicans Hsp90 gene varied between Malaysian and Iranian subjects, representing the efficacy of geographical and thermal conditions on virulence gene expression. Penerbit Universiti Sains Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55525/1/Differential%20gene%20expression%20of%20heat%20shock%20protein%2090%20%28Hsp90%29%20of%20Candida%20Albicans%20.pdf Khalili, Vajihe and Shokri, Hojjatollah and Md Akim, Abdah and Khosravi, Ali Reza (2016) Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients. Malaysian Journal of Medical Sciences, 23 (3). pp. 64-71. ISSN 1394-195X; ESSN: 2180-4303 |
| spellingShingle | Khalili, Vajihe Shokri, Hojjatollah Md Akim, Abdah Khosravi, Ali Reza Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title | Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title_full | Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title_fullStr | Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title_full_unstemmed | Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title_short | Differential gene expression of heat shock protein 90 (Hsp90) of Candida albicans obtained from Malaysian and Iranian patients |
| title_sort | differential gene expression of heat shock protein 90 (hsp90) of candida albicans obtained from malaysian and iranian patients |
| url | http://psasir.upm.edu.my/id/eprint/55525/ http://psasir.upm.edu.my/id/eprint/55525/1/Differential%20gene%20expression%20of%20heat%20shock%20protein%2090%20%28Hsp90%29%20of%20Candida%20Albicans%20.pdf |