Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55462/ http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf |
| _version_ | 1848852809316827136 |
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| author | Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Mat Said, Mohd Hazani Wasoh, Helmi |
| author_facet | Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Mat Said, Mohd Hazani Wasoh, Helmi |
| author_sort | Che-Engku-Chik, Che Engku Noramalina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to be used in robust applications. Even with the advancement in medication, the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the only technique remained as the gold standard method is the culturing method, which is the Acid Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes in one shot has raised interest among researchers, because it can skip the process of gel electrophoresis. Recently, researchers have been working on electrochemical DNA sensors which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing requirements to diagnose TB. |
| first_indexed | 2025-11-15T10:43:59Z |
| format | Article |
| id | upm-55462 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:43:59Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-554622017-09-14T09:52:52Z http://psasir.upm.edu.my/id/eprint/55462/ Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Mat Said, Mohd Hazani Wasoh, Helmi Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to be used in robust applications. Even with the advancement in medication, the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the only technique remained as the gold standard method is the culturing method, which is the Acid Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes in one shot has raised interest among researchers, because it can skip the process of gel electrophoresis. Recently, researchers have been working on electrochemical DNA sensors which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing requirements to diagnose TB. Hibiscus Publisher 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf Che-Engku-Chik, Che Engku Noramalina and Yusof, Nor Azah and Abdullah, Jaafar and Othman, Siti Sarah and Mat Said, Mohd Hazani and Wasoh, Helmi (2016) Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review. Journal of Biochemistry, Microbiology and Biotechnology, 4 (2). pp. 16-21. ISSN 2289-5779 http://journal.hibiscuspublisher.com/index.php/JOBIMB/index |
| spellingShingle | Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Mat Said, Mohd Hazani Wasoh, Helmi Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title_full | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title_fullStr | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title_full_unstemmed | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title_short | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review |
| title_sort | detection of tuberculosis (tb) using gold standard method, direct sputum smears microscopy, pcr, qpcr and electrochemical dna sensor: a mini review |
| url | http://psasir.upm.edu.my/id/eprint/55462/ http://psasir.upm.edu.my/id/eprint/55462/ http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf |