Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense
To identify the phenazine antibiotics from the selected UPMP3 strain of Pseudomonas aeruginosa and assessment of its antagonistic activity of Ganoderma boninense, three antibiotics phenazine (PHZ), phenazine-l-carboxylic acid (PCA) and pyocyanin (PYO) were extracted from the bacterial fermented brot...
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| Format: | Article |
| Language: | English |
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Friends Science Publishers
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55461/ http://psasir.upm.edu.my/id/eprint/55461/1/Detection%20of%20Phenazines.pdf |
| _version_ | 1848852809060974592 |
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| author | Parvin, Waheeda Othman, Radziah Jaafar, Hawa Wong, Mui-Yun |
| author_facet | Parvin, Waheeda Othman, Radziah Jaafar, Hawa Wong, Mui-Yun |
| author_sort | Parvin, Waheeda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To identify the phenazine antibiotics from the selected UPMP3 strain of Pseudomonas aeruginosa and assessment of its antagonistic activity of Ganoderma boninense, three antibiotics phenazine (PHZ), phenazine-l-carboxylic acid (PCA) and pyocyanin (PYO) were extracted from the bacterial fermented broth using benzene and chloroform and detected through High-performance liquid chromatography (HPLC). The effects of three antibiotics on suppressing mycelial growth of G. boninense were assayed. The maximum amount of PHZ, PCA and PYO were recorded as 1.36, 9.62 and 15.48 μg/mL, respectively and showed in vitro antagonistic activity against G. boninense. Phenazine was found to be more effective than PCA and PYO to inhibit the mycelial growth of G. boninense. It was 100% for PHZ at 1.0 mg/mL, while 78.61% for PCA and 91.87% for PYO after 7 days of incubation. Phenazine, PCA and PYO were identified as first time from oil palm rhizospheric UPMP3 strain of P. aeruginosa. The results indicated that the identified PHZ, PCA and PYO compound had good antifungal activity against G. boninense, however the PHZ can be developed as an ideal biofungicide for the control basal stem rot disease of oil palm and helps to overcome the concerns about the residual effects of synthetic fungicides. |
| first_indexed | 2025-11-15T10:43:58Z |
| format | Article |
| id | upm-55461 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:43:58Z |
| publishDate | 2016 |
| publisher | Friends Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-554612017-09-14T09:52:53Z http://psasir.upm.edu.my/id/eprint/55461/ Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense Parvin, Waheeda Othman, Radziah Jaafar, Hawa Wong, Mui-Yun To identify the phenazine antibiotics from the selected UPMP3 strain of Pseudomonas aeruginosa and assessment of its antagonistic activity of Ganoderma boninense, three antibiotics phenazine (PHZ), phenazine-l-carboxylic acid (PCA) and pyocyanin (PYO) were extracted from the bacterial fermented broth using benzene and chloroform and detected through High-performance liquid chromatography (HPLC). The effects of three antibiotics on suppressing mycelial growth of G. boninense were assayed. The maximum amount of PHZ, PCA and PYO were recorded as 1.36, 9.62 and 15.48 μg/mL, respectively and showed in vitro antagonistic activity against G. boninense. Phenazine was found to be more effective than PCA and PYO to inhibit the mycelial growth of G. boninense. It was 100% for PHZ at 1.0 mg/mL, while 78.61% for PCA and 91.87% for PYO after 7 days of incubation. Phenazine, PCA and PYO were identified as first time from oil palm rhizospheric UPMP3 strain of P. aeruginosa. The results indicated that the identified PHZ, PCA and PYO compound had good antifungal activity against G. boninense, however the PHZ can be developed as an ideal biofungicide for the control basal stem rot disease of oil palm and helps to overcome the concerns about the residual effects of synthetic fungicides. Friends Science Publishers 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55461/1/Detection%20of%20Phenazines.pdf Parvin, Waheeda and Othman, Radziah and Jaafar, Hawa and Wong, Mui-Yun (2016) Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense. International Journal of Agriculture and Biology, 18 (3). pp. 483-488. ISSN 1560-8530; ESSN: 1814-9596 10.17957/IJAB/15.0111 |
| spellingShingle | Parvin, Waheeda Othman, Radziah Jaafar, Hawa Wong, Mui-Yun Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title | Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title_full | Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title_fullStr | Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title_full_unstemmed | Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title_short | Detection of phenazines from UPMP3 strain of Pseudomonas aeruginosa and its antagonistic effects against Ganoderma boninense |
| title_sort | detection of phenazines from upmp3 strain of pseudomonas aeruginosa and its antagonistic effects against ganoderma boninense |
| url | http://psasir.upm.edu.my/id/eprint/55461/ http://psasir.upm.edu.my/id/eprint/55461/ http://psasir.upm.edu.my/id/eprint/55461/1/Detection%20of%20Phenazines.pdf |