Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb.
Objective: To isolate compounds from the roots of Garcinia cowa and to evaluated their cytotoxic activity against breast (MCF-7), prostate (DU-145), and lung (H-460) cell lines. Materials and Methods: The ground air-dried root was sequentially macerated with hexane, dichloromethane (DCM), ethyl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer - Medknow Publications and Media
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55416/ http://psasir.upm.edu.my/id/eprint/55416/1/Cytotoxic%20properties%20and%20complete%20nuclear%20magnetic%20resonance%20assignment%20of%20isolated%20.pdf |
| _version_ | 1848852796469673984 |
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| author | Wahyuni, Fatma Sri Shaari, Khozirah Stanslas, Johnson Lajis, Md. Nordin Hamidi, Dachriyanus |
| author_facet | Wahyuni, Fatma Sri Shaari, Khozirah Stanslas, Johnson Lajis, Md. Nordin Hamidi, Dachriyanus |
| author_sort | Wahyuni, Fatma Sri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To isolate compounds from the roots of Garcinia cowa and to evaluated their cytotoxic activity against breast (MCF-7), prostate (DU-145), and lung (H-460) cell lines.
Materials and Methods: The ground air-dried root was sequentially macerated with hexane, dichloromethane (DCM), ethyl acetate (EtOAc), and methanol. The DCM soluble extract was fractionated by vacuum liquid chromatography, column chromatography, and radial chromatography over silica gel with hexane, EtOAc and methanol as eluent in progressively increasing polarity manner; to yield three compounds. Their structures were elucidated based on their spectroscopic data and their comparison with those of the literature. The cytotoxicity of isolated compounds was carried out against human cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. The extract was added at various concentrations (0.1, 1, 10 and 100 mg/ml). The level of cytotoxicity was determined by calculating the level of IC50 that was based on the percentage of the cell death following the 24 h incubation with the extract.
Results: Phytochemical study on the roots of G. cowa yielded rubraxanthone (3), cowanine (4) and 1,5-dihydroxyxanthone (5). Compound 4 with an IC50 value of 4.1 ± 1.0 μM, 5.4 ± 2.3 μM and 11.3 ± 10.0 μM against MCF-7, H-460, and DU-145, respectively while compound 3 was found to be in active.
Conclusion: The results indicate that G. cowa roots could be important sources of natural cytotoxic compounds. |
| first_indexed | 2025-11-15T10:43:46Z |
| format | Article |
| id | upm-55416 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:43:46Z |
| publishDate | 2016 |
| publisher | Wolters Kluwer - Medknow Publications and Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-554162019-10-21T01:38:18Z http://psasir.upm.edu.my/id/eprint/55416/ Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. Wahyuni, Fatma Sri Shaari, Khozirah Stanslas, Johnson Lajis, Md. Nordin Hamidi, Dachriyanus Objective: To isolate compounds from the roots of Garcinia cowa and to evaluated their cytotoxic activity against breast (MCF-7), prostate (DU-145), and lung (H-460) cell lines. Materials and Methods: The ground air-dried root was sequentially macerated with hexane, dichloromethane (DCM), ethyl acetate (EtOAc), and methanol. The DCM soluble extract was fractionated by vacuum liquid chromatography, column chromatography, and radial chromatography over silica gel with hexane, EtOAc and methanol as eluent in progressively increasing polarity manner; to yield three compounds. Their structures were elucidated based on their spectroscopic data and their comparison with those of the literature. The cytotoxicity of isolated compounds was carried out against human cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. The extract was added at various concentrations (0.1, 1, 10 and 100 mg/ml). The level of cytotoxicity was determined by calculating the level of IC50 that was based on the percentage of the cell death following the 24 h incubation with the extract. Results: Phytochemical study on the roots of G. cowa yielded rubraxanthone (3), cowanine (4) and 1,5-dihydroxyxanthone (5). Compound 4 with an IC50 value of 4.1 ± 1.0 μM, 5.4 ± 2.3 μM and 11.3 ± 10.0 μM against MCF-7, H-460, and DU-145, respectively while compound 3 was found to be in active. Conclusion: The results indicate that G. cowa roots could be important sources of natural cytotoxic compounds. Wolters Kluwer - Medknow Publications and Media 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55416/1/Cytotoxic%20properties%20and%20complete%20nuclear%20magnetic%20resonance%20assignment%20of%20isolated%20.pdf Wahyuni, Fatma Sri and Shaari, Khozirah and Stanslas, Johnson and Lajis, Md. Nordin and Hamidi, Dachriyanus (2016) Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. Pharmacognosy Magazine, 12 (45). pp. 52-56. ISSN 0973-1296; ESSN: 0976-4062 10.4103/0973-1296.176115 |
| spellingShingle | Wahyuni, Fatma Sri Shaari, Khozirah Stanslas, Johnson Lajis, Md. Nordin Hamidi, Dachriyanus Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title | Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title_full | Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title_fullStr | Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title_full_unstemmed | Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title_short | Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of Garcinia cowa Roxb. |
| title_sort | cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of garcinia cowa roxb. |
| url | http://psasir.upm.edu.my/id/eprint/55416/ http://psasir.upm.edu.my/id/eprint/55416/ http://psasir.upm.edu.my/id/eprint/55416/1/Cytotoxic%20properties%20and%20complete%20nuclear%20magnetic%20resonance%20assignment%20of%20isolated%20.pdf |