Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia

Sixty-three local white-rot fungi were isolated from soil and wood samples on potato dextrose agar (PDA). All these isolates were screened for their ability to degrade 4 textile azo dyes;Ponceau 2R (C.I. 16450), Orange G (C.I. 16230), Direct Blue 71 (C.I. 34140) and Biebrich Scarlet (C.I. 26905). O...

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Main Authors: Cheng, W. N., Sim, H. K., Ahmad, Siti Aqlima, Syed, Mohd. Arif, Abd Shukor, Mohd Yunus, Yusof, Mohd. Termizi
Format: Article
Language:English
Published: Guizhou Academy of Agricultural Sciences 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55261/
http://psasir.upm.edu.my/id/eprint/55261/1/Characterization%20of%20an%20azo.pdf
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author Cheng, W. N.
Sim, H. K.
Ahmad, Siti Aqlima
Syed, Mohd. Arif
Abd Shukor, Mohd Yunus
Yusof, Mohd. Termizi
author_facet Cheng, W. N.
Sim, H. K.
Ahmad, Siti Aqlima
Syed, Mohd. Arif
Abd Shukor, Mohd Yunus
Yusof, Mohd. Termizi
author_sort Cheng, W. N.
building UPM Institutional Repository
collection Online Access
description Sixty-three local white-rot fungi were isolated from soil and wood samples on potato dextrose agar (PDA). All these isolates were screened for their ability to degrade 4 textile azo dyes;Ponceau 2R (C.I. 16450), Orange G (C.I. 16230), Direct Blue 71 (C.I. 34140) and Biebrich Scarlet (C.I. 26905). Out of 40 isolates that gave positive results, only 1 promising isolate which completely degrades all 4 dyes in the minimum amount of time was selected for further investigation. This isolate was sourced from University Putra Malaysia (UPM) Serdang campus.The isolate was tentatively identified as Coriolopsis sp. Strain arf5 based on the analysis of the internal transcribed spacer (ITS) region. Nutritional studies on defined solid medium showed that this isolate was only able to degrade the 4 azo dyes under nitrogen-limiting conditions and an additional carbon source (glucose) need to be added to provide sufficient energy for the degradation to occur. Various parameters were optimized.
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publisher Guizhou Academy of Agricultural Sciences
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spelling upm-552612018-01-22T08:28:32Z http://psasir.upm.edu.my/id/eprint/55261/ Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia Cheng, W. N. Sim, H. K. Ahmad, Siti Aqlima Syed, Mohd. Arif Abd Shukor, Mohd Yunus Yusof, Mohd. Termizi Sixty-three local white-rot fungi were isolated from soil and wood samples on potato dextrose agar (PDA). All these isolates were screened for their ability to degrade 4 textile azo dyes;Ponceau 2R (C.I. 16450), Orange G (C.I. 16230), Direct Blue 71 (C.I. 34140) and Biebrich Scarlet (C.I. 26905). Out of 40 isolates that gave positive results, only 1 promising isolate which completely degrades all 4 dyes in the minimum amount of time was selected for further investigation. This isolate was sourced from University Putra Malaysia (UPM) Serdang campus.The isolate was tentatively identified as Coriolopsis sp. Strain arf5 based on the analysis of the internal transcribed spacer (ITS) region. Nutritional studies on defined solid medium showed that this isolate was only able to degrade the 4 azo dyes under nitrogen-limiting conditions and an additional carbon source (glucose) need to be added to provide sufficient energy for the degradation to occur. Various parameters were optimized. Guizhou Academy of Agricultural Sciences 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55261/1/Characterization%20of%20an%20azo.pdf Cheng, W. N. and Sim, H. K. and Ahmad, Siti Aqlima and Syed, Mohd. Arif and Abd Shukor, Mohd Yunus and Yusof, Mohd. Termizi (2016) Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia. Mycosphere, 7 (5). pp. 560-569. ISSN 2077-7000; ESSN: 2077-7019 http://www.mycosphere.org 10.5943/mycosphere/7/5/3
spellingShingle Cheng, W. N.
Sim, H. K.
Ahmad, Siti Aqlima
Syed, Mohd. Arif
Abd Shukor, Mohd Yunus
Yusof, Mohd. Termizi
Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title_full Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title_fullStr Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title_full_unstemmed Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title_short Characterization of an azo-dye-degrading white rot fungus isolated from Malaysia
title_sort characterization of an azo-dye-degrading white rot fungus isolated from malaysia
url http://psasir.upm.edu.my/id/eprint/55261/
http://psasir.upm.edu.my/id/eprint/55261/
http://psasir.upm.edu.my/id/eprint/55261/
http://psasir.upm.edu.my/id/eprint/55261/1/Characterization%20of%20an%20azo.pdf