Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells
The present study is aimed at optimizing the in vitro culture protocol for generation of rat bone marrow- (BM-) derived mesenchymal stem cells (MSCs) and characterizing the culture-mediated cellular senescence. The initial phase of generation and characterization was conducted using the adherent cel...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55258/ http://psasir.upm.edu.my/id/eprint/55258/1/Characterization%20and%20expression%20of%20senescence%20marker%20in%20prolonged%20passages%20of%20rat%20bone%20marrow.pdf |
| _version_ | 1848852755300483072 |
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| author | Ridzuan, Noridzzaida Al Abbar, Akram Wai, Kien Yip Maqbool, Maryam Ramasamy, Rajesh |
| author_facet | Ridzuan, Noridzzaida Al Abbar, Akram Wai, Kien Yip Maqbool, Maryam Ramasamy, Rajesh |
| author_sort | Ridzuan, Noridzzaida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study is aimed at optimizing the in vitro culture protocol for generation of rat bone marrow- (BM-) derived mesenchymal stem cells (MSCs) and characterizing the culture-mediated cellular senescence. The initial phase of generation and characterization was conducted using the adherent cells from Sprague Dawley (SD) rat’s BM via morphological analysis, growth kinetics, colony forming unit capacity, immunophenotyping, and mesodermal lineage differentiation. Mesenchymal stem cells were successfully generated and characterized as delineated by the expressions of CD90.1, CD44H, CD29, and CD71 and lack of CD11b/c and CD45 markers. Upon induction, rBM-MSCs differentiated into osteocytes and adipocytes and expressed osteocytes and adipocytes genes. However, a decline in cell growth was observed at passage 4 onwards and it was further deciphered through apoptosis, cell cycle, and senescence assays. Despite the enhanced cell viability at later passages (P4-5), the expression of senescence marker, β-galactosidase, was significantly increased at passage 5. Furthermore, the cell cycle analysis has confirmed the in vitro culture-mediated cellular senescence where cells were arrested at the G0/G1 phase of cell cycle. Although the currently optimized protocols had successfully yielded rBM-MSCs, the culture-mediated cellular senescence limits the growth of rBM-MSCs and its potential use in rat-based MSC research. |
| first_indexed | 2025-11-15T10:43:07Z |
| format | Article |
| id | upm-55258 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:43:07Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-552582018-01-22T08:35:49Z http://psasir.upm.edu.my/id/eprint/55258/ Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells Ridzuan, Noridzzaida Al Abbar, Akram Wai, Kien Yip Maqbool, Maryam Ramasamy, Rajesh The present study is aimed at optimizing the in vitro culture protocol for generation of rat bone marrow- (BM-) derived mesenchymal stem cells (MSCs) and characterizing the culture-mediated cellular senescence. The initial phase of generation and characterization was conducted using the adherent cells from Sprague Dawley (SD) rat’s BM via morphological analysis, growth kinetics, colony forming unit capacity, immunophenotyping, and mesodermal lineage differentiation. Mesenchymal stem cells were successfully generated and characterized as delineated by the expressions of CD90.1, CD44H, CD29, and CD71 and lack of CD11b/c and CD45 markers. Upon induction, rBM-MSCs differentiated into osteocytes and adipocytes and expressed osteocytes and adipocytes genes. However, a decline in cell growth was observed at passage 4 onwards and it was further deciphered through apoptosis, cell cycle, and senescence assays. Despite the enhanced cell viability at later passages (P4-5), the expression of senescence marker, β-galactosidase, was significantly increased at passage 5. Furthermore, the cell cycle analysis has confirmed the in vitro culture-mediated cellular senescence where cells were arrested at the G0/G1 phase of cell cycle. Although the currently optimized protocols had successfully yielded rBM-MSCs, the culture-mediated cellular senescence limits the growth of rBM-MSCs and its potential use in rat-based MSC research. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55258/1/Characterization%20and%20expression%20of%20senescence%20marker%20in%20prolonged%20passages%20of%20rat%20bone%20marrow.pdf Ridzuan, Noridzzaida and Al Abbar, Akram and Wai, Kien Yip and Maqbool, Maryam and Ramasamy, Rajesh (2016) Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells. Stem Cell International. art. no. 8487264. pp. 1-14. ISSN 1687-9678 10.1155/2016/8487264 |
| spellingShingle | Ridzuan, Noridzzaida Al Abbar, Akram Wai, Kien Yip Maqbool, Maryam Ramasamy, Rajesh Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title | Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title_full | Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title_fullStr | Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title_full_unstemmed | Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title_short | Characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| title_sort | characterization and expression of senescence marker in prolonged passages of rat bone marrow-derived mesenchymal stem cells |
| url | http://psasir.upm.edu.my/id/eprint/55258/ http://psasir.upm.edu.my/id/eprint/55258/ http://psasir.upm.edu.my/id/eprint/55258/1/Characterization%20and%20expression%20of%20senescence%20marker%20in%20prolonged%20passages%20of%20rat%20bone%20marrow.pdf |