Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae
A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isola...
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| Format: | Article |
| Language: | English |
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Scientific Research Publishing
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55200/ http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf |
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| author | Awla, Hayman Kakakhan Kadir, Jugah Othman, Radziah Rashid, Tavga Sulaiman Wong, Mui Yun |
| author_facet | Awla, Hayman Kakakhan Kadir, Jugah Othman, Radziah Rashid, Tavga Sulaiman Wong, Mui Yun |
| author_sort | Awla, Hayman Kakakhan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications. |
| first_indexed | 2025-11-15T10:42:52Z |
| format | Article |
| id | upm-55200 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:52Z |
| publishDate | 2016 |
| publisher | Scientific Research Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-552002018-01-22T10:16:16Z http://psasir.upm.edu.my/id/eprint/55200/ Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae Awla, Hayman Kakakhan Kadir, Jugah Othman, Radziah Rashid, Tavga Sulaiman Wong, Mui Yun A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine. These compounds had good general antifungal activity and might have potential future agricultural applications. Scientific Research Publishing 2016-05 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf Awla, Hayman Kakakhan and Kadir, Jugah and Othman, Radziah and Rashid, Tavga Sulaiman and Wong, Mui Yun (2016) Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae. American Journal of Plant Sciences, 7 (7). art. no. 66540. pp. 1077-1085. ISSN 2158-2742; ESSN: 2158-2750 http://www.scirp.org/journal/ajps 10.4236/ajps.2016.77103 |
| spellingShingle | Awla, Hayman Kakakhan Kadir, Jugah Othman, Radziah Rashid, Tavga Sulaiman Wong, Mui Yun Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title | Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title_full | Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title_fullStr | Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title_full_unstemmed | Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title_short | Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae |
| title_sort | bioactive compounds produced by streptomyces sp. isolate upmrs4 and antifungal activity against pyricularia oryzae |
| url | http://psasir.upm.edu.my/id/eprint/55200/ http://psasir.upm.edu.my/id/eprint/55200/ http://psasir.upm.edu.my/id/eprint/55200/ http://psasir.upm.edu.my/id/eprint/55200/1/Bioactive%20compounds%20produced%20by%20Streptomyces%20sp.%20isolate%20UPMRS4%20and%20antifungal%20activity%20against%20Pyricularia%20oryzae.pdf |