Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells
Pharmocological relevance: 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA), is a phloroglucinol compound found naturally in Melicope ptelefolia. Melicope ptelefolia has been used traditionally for centuries as natural remedy for wound infections and inflammatory diseases. Aim of the study: Endothe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55186/ http://psasir.upm.edu.my/id/eprint/55186/1/Barrier%20protective%20effects%20of%202%2C4%2C6-trihydroxy-3-geranyl%20acetophenone%20on%20lipopolysaccharides-stimulated%20inflammatory%20responses%20in%20human%20umbilical%20vein%20endothelial%20cells.pdf |
| _version_ | 1848852736141950976 |
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| author | Yi, Joong Chong Musa, Nazmi Firdaus Ng, Chean Hui Shaari, Khozirah Israf, Daud Ahmad Chau, Ling Tham |
| author_facet | Yi, Joong Chong Musa, Nazmi Firdaus Ng, Chean Hui Shaari, Khozirah Israf, Daud Ahmad Chau, Ling Tham |
| author_sort | Yi, Joong Chong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pharmocological relevance: 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA), is a phloroglucinol compound found naturally in Melicope ptelefolia. Melicope ptelefolia has been used traditionally for centuries as natural remedy for wound infections and inflammatory diseases.
Aim of the study: Endothelial barrier dysfunction is a pathological hallmark of many diseases and can be caused by lipopolysaccharides (LPS) stimulation. Therefore, this study aims to investigate the possible barrier protective effects of tHGA upon LPS-stimulated inflammatory responses in human umbilical vein endothelial cells (HUVECs).
Materials and methods: HUVECs were pretreated with tHGA prior to LPS stimulation, where inflammatory parameters including permeability, monocyte adhesion and migration, and release of pro-inflammatory mediators were examined. Additionally, the effect of tHGA on F-actin rearrangement and adhesion protein expression of LPS-stimulated HUVECs was evaluated.
Results: It was found that pretreatment with tHGA inhibited monocyte adhesion and transendothelial migration, reduced endothelial hyperpermeability and secretion of prostaglandin E2 (PGE2). Additionally, tHGA inhibited cytoskeletal rearrangement and adhesion protein expression on LPS-stimulated HUVECs.
Conclusion: As the regulation of endothelial barrier dysfunction can be one of the therapeutic strategies to improve the outcome of inflammation, tHGA may be able to preserve vascular barrier integrity of endothelial cells following LPS-stimulated dysfunction, thereby endorsing its potential usefulness in vascular inflammatory diseases. |
| first_indexed | 2025-11-15T10:42:49Z |
| format | Article |
| id | upm-55186 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:49Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-551862017-12-07T06:26:26Z http://psasir.upm.edu.my/id/eprint/55186/ Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells Yi, Joong Chong Musa, Nazmi Firdaus Ng, Chean Hui Shaari, Khozirah Israf, Daud Ahmad Chau, Ling Tham Pharmocological relevance: 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA), is a phloroglucinol compound found naturally in Melicope ptelefolia. Melicope ptelefolia has been used traditionally for centuries as natural remedy for wound infections and inflammatory diseases. Aim of the study: Endothelial barrier dysfunction is a pathological hallmark of many diseases and can be caused by lipopolysaccharides (LPS) stimulation. Therefore, this study aims to investigate the possible barrier protective effects of tHGA upon LPS-stimulated inflammatory responses in human umbilical vein endothelial cells (HUVECs). Materials and methods: HUVECs were pretreated with tHGA prior to LPS stimulation, where inflammatory parameters including permeability, monocyte adhesion and migration, and release of pro-inflammatory mediators were examined. Additionally, the effect of tHGA on F-actin rearrangement and adhesion protein expression of LPS-stimulated HUVECs was evaluated. Results: It was found that pretreatment with tHGA inhibited monocyte adhesion and transendothelial migration, reduced endothelial hyperpermeability and secretion of prostaglandin E2 (PGE2). Additionally, tHGA inhibited cytoskeletal rearrangement and adhesion protein expression on LPS-stimulated HUVECs. Conclusion: As the regulation of endothelial barrier dysfunction can be one of the therapeutic strategies to improve the outcome of inflammation, tHGA may be able to preserve vascular barrier integrity of endothelial cells following LPS-stimulated dysfunction, thereby endorsing its potential usefulness in vascular inflammatory diseases. Elsevier 2016-11-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55186/1/Barrier%20protective%20effects%20of%202%2C4%2C6-trihydroxy-3-geranyl%20acetophenone%20on%20lipopolysaccharides-stimulated%20inflammatory%20responses%20in%20human%20umbilical%20vein%20endothelial%20cells.pdf Yi, Joong Chong and Musa, Nazmi Firdaus and Ng, Chean Hui and Shaari, Khozirah and Israf, Daud Ahmad and Chau, Ling Tham (2016) Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells. Journal of Ethnopharmacology, 192. pp. 248-255. ISSN 0378-8741; ESSN: 1872-7573 http://www.elsevier.com/locate/issn/03788741 10.1016/j.jep.2016.07.032 |
| spellingShingle | Yi, Joong Chong Musa, Nazmi Firdaus Ng, Chean Hui Shaari, Khozirah Israf, Daud Ahmad Chau, Ling Tham Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title | Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title_full | Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title_fullStr | Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title_full_unstemmed | Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title_short | Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| title_sort | barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells |
| url | http://psasir.upm.edu.my/id/eprint/55186/ http://psasir.upm.edu.my/id/eprint/55186/ http://psasir.upm.edu.my/id/eprint/55186/ http://psasir.upm.edu.my/id/eprint/55186/1/Barrier%20protective%20effects%20of%202%2C4%2C6-trihydroxy-3-geranyl%20acetophenone%20on%20lipopolysaccharides-stimulated%20inflammatory%20responses%20in%20human%20umbilical%20vein%20endothelial%20cells.pdf |