Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10
An aqueous two-phase flotation (ATPF) system based on polyethylene glycol (PEG) and sodium citrate (NaNO3C6H5O7·2H2O) was considered for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. The effects of ATPF parameters namely phase composition, tie-l...
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| Format: | Article |
| Language: | English |
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Elsevier BV
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55111/ http://psasir.upm.edu.my/id/eprint/55111/1/Aqueous%20two-phase%20flotation%20for%20primary%20recovery%20of%20bacteriocin-like%20inhibitory%20substance%20%28BLIS%29%20from%20Pediococcus%20acidilactici%20Kp10.pdf |
| _version_ | 1848852715456692224 |
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| author | Md Sidek, Nurul Lyana Joo, Shun Tan Abbasiliasi, Sahar Faizal Wong, Fadzlie Wong Mustafa, Shuhaimi Ariff, Arbakariya |
| author_facet | Md Sidek, Nurul Lyana Joo, Shun Tan Abbasiliasi, Sahar Faizal Wong, Fadzlie Wong Mustafa, Shuhaimi Ariff, Arbakariya |
| author_sort | Md Sidek, Nurul Lyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An aqueous two-phase flotation (ATPF) system based on polyethylene glycol (PEG) and sodium citrate (NaNO3C6H5O7·2H2O) was considered for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. The effects of ATPF parameters namely phase composition, tie-line length (TLL), volume ratio between the two phases (VR), amount of crude load (CL), pH, nitrogen gas flow rate (FR) and flotation time (FT) on the performance of recovery were evaluated. BLIS was mainly concentrated into the upper PEG-rich phase in all systems tested so far. The optimum conditions for BLIS purification, which composed of PEG 8000/sodium citrate, were: TLL of 42.6, VR of 0.4, CL of 22% (w/w), pH 7, average FT of 30min and FR of 20mL/min. BLIS was partially purified up to 5.9-fold with a separation efficiency of 99% under this optimal conditions. A maximum yield of BLIS activity of about 70.3% was recovered in the PEG phase. The BLIS from the top phase was successfully recovered with a single band in SDS-gel with molecular weight of about 10-15kDa. ATPF was found to be an effective technique for the recovery of BLIS from the fermentation broth of P. acidilactici Kp10. |
| first_indexed | 2025-11-15T10:42:29Z |
| format | Article |
| id | upm-55111 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:29Z |
| publishDate | 2016 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-551112017-12-26T09:50:31Z http://psasir.upm.edu.my/id/eprint/55111/ Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 Md Sidek, Nurul Lyana Joo, Shun Tan Abbasiliasi, Sahar Faizal Wong, Fadzlie Wong Mustafa, Shuhaimi Ariff, Arbakariya An aqueous two-phase flotation (ATPF) system based on polyethylene glycol (PEG) and sodium citrate (NaNO3C6H5O7·2H2O) was considered for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. The effects of ATPF parameters namely phase composition, tie-line length (TLL), volume ratio between the two phases (VR), amount of crude load (CL), pH, nitrogen gas flow rate (FR) and flotation time (FT) on the performance of recovery were evaluated. BLIS was mainly concentrated into the upper PEG-rich phase in all systems tested so far. The optimum conditions for BLIS purification, which composed of PEG 8000/sodium citrate, were: TLL of 42.6, VR of 0.4, CL of 22% (w/w), pH 7, average FT of 30min and FR of 20mL/min. BLIS was partially purified up to 5.9-fold with a separation efficiency of 99% under this optimal conditions. A maximum yield of BLIS activity of about 70.3% was recovered in the PEG phase. The BLIS from the top phase was successfully recovered with a single band in SDS-gel with molecular weight of about 10-15kDa. ATPF was found to be an effective technique for the recovery of BLIS from the fermentation broth of P. acidilactici Kp10. Elsevier BV 2016-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55111/1/Aqueous%20two-phase%20flotation%20for%20primary%20recovery%20of%20bacteriocin-like%20inhibitory%20substance%20%28BLIS%29%20from%20Pediococcus%20acidilactici%20Kp10.pdf Md Sidek, Nurul Lyana and Joo, Shun Tan and Abbasiliasi, Sahar and Faizal Wong, Fadzlie Wong and Mustafa, Shuhaimi and Ariff, Arbakariya (2016) Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 1027. pp. 81-87. ISSN 1570-0232; ESSN: 1873-376X http://www.elsevier.com/locate/issn/15700232 10.1016/j.jchromb.2016.05.024 |
| spellingShingle | Md Sidek, Nurul Lyana Joo, Shun Tan Abbasiliasi, Sahar Faizal Wong, Fadzlie Wong Mustafa, Shuhaimi Ariff, Arbakariya Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title | Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title_full | Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title_fullStr | Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title_full_unstemmed | Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title_short | Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10 |
| title_sort | aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (blis) from pediococcus acidilactici kp10 |
| url | http://psasir.upm.edu.my/id/eprint/55111/ http://psasir.upm.edu.my/id/eprint/55111/ http://psasir.upm.edu.my/id/eprint/55111/ http://psasir.upm.edu.my/id/eprint/55111/1/Aqueous%20two-phase%20flotation%20for%20primary%20recovery%20of%20bacteriocin-like%20inhibitory%20substance%20%28BLIS%29%20from%20Pediococcus%20acidilactici%20Kp10.pdf |