An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense
Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Tric...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Verlag
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/55028/ http://psasir.upm.edu.my/id/eprint/55028/1/An%20in%20vitro%20study%20of%20the%20antifungal%20activity%20of%20Trichoderma%20virens%207b%20and%20a%20profile%20of%20its%20.pdf |
| _version_ | 1848852696198545408 |
|---|---|
| author | Lee, Pei Lee Angel Yusof, Mohd Termizi Ismail, Intan Safinar Tay, Bonnie Yen Ping Mohamed Azni, Intan Nur Ainni Kamarudin, Norman Hj Sundram, Shamala |
| author_facet | Lee, Pei Lee Angel Yusof, Mohd Termizi Ismail, Intan Safinar Tay, Bonnie Yen Ping Mohamed Azni, Intan Nur Ainni Kamarudin, Norman Hj Sundram, Shamala |
| author_sort | Lee, Pei Lee Angel |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G. boninense by identifying and characterizing the chemical compounds as well as the physical mechanisms by T. virens 7b. Hexane extract of the isolate gave 62.60% ± 6.41 inhibition against G. boninense and observation under scanning electron microscope (SEM) detected severe mycelial deformation of the pathogen at the region of inhibition. Similar mycelia deformation of G. boninense was observed with a fungicide treatment, Benlate® indicating comparable fungicidal effect by T. virens 7b. Fraction 4 and 5 of hexane active fractions through preparative thin layer chromatography (P-TLC) was identified giving the best inhibition of the pathogen. These fractions comprised of ketones, alcohols, aldehydes, lactones, sesquiterpenes, monoterpenes, sulphides, and free fatty acids profiled through gas chromatography mass spectrometry detector (GC/MSD). A novel antifungal compound discovery of phenylethyl alcohol (PEA) by T. virens 7b is reported through this study. T. virens 7b also proved to be an active siderophore producer through chrome azurol S (CAS) agar assay. The study demonstrated the possible mechanisms involved and responsible in the successful inhibition of G. boninense. |
| first_indexed | 2025-11-15T10:42:11Z |
| format | Article |
| id | upm-55028 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:11Z |
| publishDate | 2016 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-550282018-07-12T11:13:49Z http://psasir.upm.edu.my/id/eprint/55028/ An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense Lee, Pei Lee Angel Yusof, Mohd Termizi Ismail, Intan Safinar Tay, Bonnie Yen Ping Mohamed Azni, Intan Nur Ainni Kamarudin, Norman Hj Sundram, Shamala Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G. boninense by identifying and characterizing the chemical compounds as well as the physical mechanisms by T. virens 7b. Hexane extract of the isolate gave 62.60% ± 6.41 inhibition against G. boninense and observation under scanning electron microscope (SEM) detected severe mycelial deformation of the pathogen at the region of inhibition. Similar mycelia deformation of G. boninense was observed with a fungicide treatment, Benlate® indicating comparable fungicidal effect by T. virens 7b. Fraction 4 and 5 of hexane active fractions through preparative thin layer chromatography (P-TLC) was identified giving the best inhibition of the pathogen. These fractions comprised of ketones, alcohols, aldehydes, lactones, sesquiterpenes, monoterpenes, sulphides, and free fatty acids profiled through gas chromatography mass spectrometry detector (GC/MSD). A novel antifungal compound discovery of phenylethyl alcohol (PEA) by T. virens 7b is reported through this study. T. virens 7b also proved to be an active siderophore producer through chrome azurol S (CAS) agar assay. The study demonstrated the possible mechanisms involved and responsible in the successful inhibition of G. boninense. Springer Verlag 2016-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/55028/1/An%20in%20vitro%20study%20of%20the%20antifungal%20activity%20of%20Trichoderma%20virens%207b%20and%20a%20profile%20of%20its%20.pdf Lee, Pei Lee Angel and Yusof, Mohd Termizi and Ismail, Intan Safinar and Tay, Bonnie Yen Ping and Mohamed Azni, Intan Nur Ainni and Kamarudin, Norman Hj and Sundram, Shamala (2016) An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense. Journal of Microbiology, 54 (11). pp. 732-744. ISSN 1225-8873; ESSN:1976-3794 10.1007/s12275-016-6304-4 |
| spellingShingle | Lee, Pei Lee Angel Yusof, Mohd Termizi Ismail, Intan Safinar Tay, Bonnie Yen Ping Mohamed Azni, Intan Nur Ainni Kamarudin, Norman Hj Sundram, Shamala An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title | An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title_full | An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title_fullStr | An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title_full_unstemmed | An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title_short | An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense |
| title_sort | in vitro study of the antifungal activity of trichoderma virens 7b and a profile of its non-polar antifungal components released against ganoderma boninense |
| url | http://psasir.upm.edu.my/id/eprint/55028/ http://psasir.upm.edu.my/id/eprint/55028/ http://psasir.upm.edu.my/id/eprint/55028/1/An%20in%20vitro%20study%20of%20the%20antifungal%20activity%20of%20Trichoderma%20virens%207b%20and%20a%20profile%20of%20its%20.pdf |