Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glyc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55006/ http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf |
| _version_ | 1848852690543575040 |
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| author | Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul |
| author_facet | Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul |
| author_sort | Ahmad, Nor Monica |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075-10 µM and 10-55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days. |
| first_indexed | 2025-11-15T10:42:05Z |
| format | Article |
| id | upm-55006 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:05Z |
| publishDate | 2016 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-550062018-07-12T10:30:35Z http://psasir.upm.edu.my/id/eprint/55006/ Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075-10 µM and 10-55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days. MDPI 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf Ahmad, Nor Monica and Abdullah, Jaafar and Yusof, Nor Azah and Ab Rashid, Ahmad Hazri and Abd Rahman, Samsulida and Hasan, Md. Rakibul (2016) Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds. Biosensors, 6 (31). pp. 1-14. ISSN 2079-6374 10.3390/bios6030031 |
| spellingShingle | Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title | Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title_full | Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title_fullStr | Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title_full_unstemmed | Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title_short | Amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| title_sort | amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds |
| url | http://psasir.upm.edu.my/id/eprint/55006/ http://psasir.upm.edu.my/id/eprint/55006/ http://psasir.upm.edu.my/id/eprint/55006/1/Amperometric%20Biosensor%20Based%20on%20Zirconium%20OxidePolyethylene%20GlycolTyrosinase%20Composite%20Film%20for%20the%20Detection%20of%20Phenolic%20Compounds.pdf |