Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey
Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54996/ http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf |
| _version_ | 1848852687164014592 |
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| author | Balasubramaniam, Vimala June, Chelyn Lee Mohd Noh, Mohd Fairunizal Ahmad, Syahida Brownlee, Iain A. Ismail, Amin |
| author_facet | Balasubramaniam, Vimala June, Chelyn Lee Mohd Noh, Mohd Fairunizal Ahmad, Syahida Brownlee, Iain A. Ismail, Amin |
| author_sort | Balasubramaniam, Vimala |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α-Amylase activity was assessed by dinitrosalicylic acid (DNS) assay, while the antioxidant property was determined by oxygen radical absorbance capacity (ORAC) analysis. The anti-inflammatory effects of the seaweed samples were evaluated by nitric oxide (NO), interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) activities on the interferon-gamma/lipopolysaccharide (IFN-γ/LPS)-stimulated murine macrophage cell line (RAW 264.7) using Griess reaction and immunoassays, respectively. At 10 mg mL−1, E. denticulatum ethanol extract and three fractions inhibited α-amylase activities at variable levels. The highest (67 %) inhibition of α-amylase enzyme was by the ethanol crude extract. The three fractions showed inhibition with a mean of 42 %. Crude ethanol extracts also exhibited higher antioxidant capacity (36,400 ± 23.5 mol Trolox equivalent (TE) (100 g)−1) when compared to the fractions. Crude extract and fractions (1–100 μg mL−1), also exhibited anti-inflammatory activity without showing any cytotoxic effect to RAW 264.7 cells. The present study suggests that E. denticulatum has the potential to be a promising source of effective functional metabolite. An extensive research on the edible varieties would contribute to a better understanding of their importance as functional food. |
| first_indexed | 2025-11-15T10:42:02Z |
| format | Article |
| id | upm-54996 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:42:02Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-549962018-07-12T09:44:09Z http://psasir.upm.edu.my/id/eprint/54996/ Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey Balasubramaniam, Vimala June, Chelyn Lee Mohd Noh, Mohd Fairunizal Ahmad, Syahida Brownlee, Iain A. Ismail, Amin Eucheuma denticulatum is a red edible seaweed that grows in the East Malaysia coastal region. The objective of this study was to investigate the α-amylase, antioxidant and anti-inflammatory activities of E. denticulatum ethanol extract and its three fractions (n-hexane, ethyl acetate and acetone). α-Amylase activity was assessed by dinitrosalicylic acid (DNS) assay, while the antioxidant property was determined by oxygen radical absorbance capacity (ORAC) analysis. The anti-inflammatory effects of the seaweed samples were evaluated by nitric oxide (NO), interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) activities on the interferon-gamma/lipopolysaccharide (IFN-γ/LPS)-stimulated murine macrophage cell line (RAW 264.7) using Griess reaction and immunoassays, respectively. At 10 mg mL−1, E. denticulatum ethanol extract and three fractions inhibited α-amylase activities at variable levels. The highest (67 %) inhibition of α-amylase enzyme was by the ethanol crude extract. The three fractions showed inhibition with a mean of 42 %. Crude ethanol extracts also exhibited higher antioxidant capacity (36,400 ± 23.5 mol Trolox equivalent (TE) (100 g)−1) when compared to the fractions. Crude extract and fractions (1–100 μg mL−1), also exhibited anti-inflammatory activity without showing any cytotoxic effect to RAW 264.7 cells. The present study suggests that E. denticulatum has the potential to be a promising source of effective functional metabolite. An extensive research on the edible varieties would contribute to a better understanding of their importance as functional food. Springer 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf Balasubramaniam, Vimala and June, Chelyn Lee and Mohd Noh, Mohd Fairunizal and Ahmad, Syahida and Brownlee, Iain A. and Ismail, Amin (2016) Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey. Journal of Applied Phycology, 28. pp. 1965-1974. ISSN 0921-8971; ESSN: 1573-5176 10.1007/s10811-015-0690-6 |
| spellingShingle | Balasubramaniam, Vimala June, Chelyn Lee Mohd Noh, Mohd Fairunizal Ahmad, Syahida Brownlee, Iain A. Ismail, Amin Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title | Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title_full | Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title_fullStr | Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title_full_unstemmed | Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title_short | Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey |
| title_sort | alpha-amylase, antioxidant, and anti-inflammatory activities of eucheuma denticulatum (n.l. burman) f.s. collins and hervey |
| url | http://psasir.upm.edu.my/id/eprint/54996/ http://psasir.upm.edu.my/id/eprint/54996/ http://psasir.upm.edu.my/id/eprint/54996/1/Alpha-amylase%2C%20antioxidant%2C%20and%20anti-inflammatory%20.pdf |