A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components
A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygal...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54887/ http://psasir.upm.edu.my/id/eprint/54887/1/A%20novel%20aqueous%20micellar%20two-phase%20system%20composed%20of%20surfactant%20and%20mannitol.pdf |
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| author | Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Hussin, Muhaini Sarker, Md. Zaidul Islam Chong, Gun Hean |
| author_facet | Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Hussin, Muhaini Sarker, Md. Zaidul Islam Chong, Gun Hean |
| author_sort | Amid, Mehrnoush |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%. |
| first_indexed | 2025-11-15T10:41:34Z |
| format | Article |
| id | upm-54887 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:41:34Z |
| publishDate | 2016 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-548872018-06-12T02:21:47Z http://psasir.upm.edu.my/id/eprint/54887/ A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Hussin, Muhaini Sarker, Md. Zaidul Islam Chong, Gun Hean A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%. Taylor & Francis 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54887/1/A%20novel%20aqueous%20micellar%20two-phase%20system%20composed%20of%20surfactant%20and%20mannitol.pdf Amid, Mehrnoush and Murshid, Fara Syazana and Manap, Mohd Yazid and Hussin, Muhaini and Sarker, Md. Zaidul Islam and Chong, Gun Hean (2016) A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components. Separation Science and Technology, 51. pp. 968-975. ISSN 0149-6395; ESSN:1520-5754 10.1080/01496395.2016.1142562 |
| spellingShingle | Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Hussin, Muhaini Sarker, Md. Zaidul Islam Chong, Gun Hean A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title | A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title_full | A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title_fullStr | A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title_full_unstemmed | A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title_short | A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from Durio zibethinus Murray and recycling phase components |
| title_sort | novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from durio zibethinus murray and recycling phase components |
| url | http://psasir.upm.edu.my/id/eprint/54887/ http://psasir.upm.edu.my/id/eprint/54887/ http://psasir.upm.edu.my/id/eprint/54887/1/A%20novel%20aqueous%20micellar%20two-phase%20system%20composed%20of%20surfactant%20and%20mannitol.pdf |