A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor
Influence of waterborne butachlor (BUC), a commonly used pesticide, on morphometric, biochemical, and molecular biomarkers was evaluated in juvenile, full sibling, diploid and triploid African catfish (Clarias gariepinus). Fish were exposed for 21 days to one of three concentrations of BUC [mean mea...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/54825/ http://psasir.upm.edu.my/id/eprint/54825/1/A%20comparison%20of%20biomarker%20responses%20in%20juvenile%20diploid%20and%20triploid%20African%20catfish%2C%20.pdf |
| _version_ | 1848852639517769728 |
|---|---|
| author | Karami, Ali Omar, Dzolkhifli Lazorchak, James M. Chee, Kong Yap Hashim, Zailina Courtenay, Simon C. |
| author_facet | Karami, Ali Omar, Dzolkhifli Lazorchak, James M. Chee, Kong Yap Hashim, Zailina Courtenay, Simon C. |
| author_sort | Karami, Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Influence of waterborne butachlor (BUC), a commonly used pesticide, on morphometric, biochemical, and molecular biomarkers was evaluated in juvenile, full sibling, diploid and triploid African catfish (Clarias gariepinus). Fish were exposed for 21 days to one of three concentrations of BUC [mean measured µg/L: 22, 44 or 60]. Unexposed (control) triploids were heavier and longer and had higher visceral-somatic index (VSI) than diploids. Also, they had lighter liver weight (HSI) and showed lower transcript levels of brain gonadotropin-releasing hormone (GnRH), aromatase (cyp191b) and fushi tarazu-factor (ftz-f1), and plasma testosterone levels than diploids. Butachlor treatments had no effects, in either diploid or triploid fish, on VSI, HSI, weight or length changes, condition factor (CF), levels of plasma testosterone, 17-β estradiol (E2), cortisol, cholesterol, or mRNA levels of brain tryptophan hydroxylase (tph2), forkhead box L2 (foxl2), and 11 β-hydroxysteroid dehydrogenase type 2 (11β-hsd2). Expressions of cyp191b and ftz-f1 in triploids were upregulated by the two highest concentrations of BUC. In diploid fish, however, exposures to all BUC concentrations decreased GnRH transcription and the medium BUC concentration decreased ftz-f1 transcription. Substantial differences between ploidies in basal biomarker responses are consistent with the reported impaired reproductive axis in triploid C. gariepinus. Furthermore, the present study showed the low impact of short term exposure to BUC on reproductive axis in C. gariepinus. |
| first_indexed | 2025-11-15T10:41:17Z |
| format | Article |
| id | upm-54825 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:41:17Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-548252018-04-04T05:30:52Z http://psasir.upm.edu.my/id/eprint/54825/ A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor Karami, Ali Omar, Dzolkhifli Lazorchak, James M. Chee, Kong Yap Hashim, Zailina Courtenay, Simon C. Influence of waterborne butachlor (BUC), a commonly used pesticide, on morphometric, biochemical, and molecular biomarkers was evaluated in juvenile, full sibling, diploid and triploid African catfish (Clarias gariepinus). Fish were exposed for 21 days to one of three concentrations of BUC [mean measured µg/L: 22, 44 or 60]. Unexposed (control) triploids were heavier and longer and had higher visceral-somatic index (VSI) than diploids. Also, they had lighter liver weight (HSI) and showed lower transcript levels of brain gonadotropin-releasing hormone (GnRH), aromatase (cyp191b) and fushi tarazu-factor (ftz-f1), and plasma testosterone levels than diploids. Butachlor treatments had no effects, in either diploid or triploid fish, on VSI, HSI, weight or length changes, condition factor (CF), levels of plasma testosterone, 17-β estradiol (E2), cortisol, cholesterol, or mRNA levels of brain tryptophan hydroxylase (tph2), forkhead box L2 (foxl2), and 11 β-hydroxysteroid dehydrogenase type 2 (11β-hsd2). Expressions of cyp191b and ftz-f1 in triploids were upregulated by the two highest concentrations of BUC. In diploid fish, however, exposures to all BUC concentrations decreased GnRH transcription and the medium BUC concentration decreased ftz-f1 transcription. Substantial differences between ploidies in basal biomarker responses are consistent with the reported impaired reproductive axis in triploid C. gariepinus. Furthermore, the present study showed the low impact of short term exposure to BUC on reproductive axis in C. gariepinus. Elsevier 2016-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54825/1/A%20comparison%20of%20biomarker%20responses%20in%20juvenile%20diploid%20and%20triploid%20African%20catfish%2C%20.pdf Karami, Ali and Omar, Dzolkhifli and Lazorchak, James M. and Chee, Kong Yap and Hashim, Zailina and Courtenay, Simon C. (2016) A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor. Environmental Research, 151. pp. 313-320. ISSN 0013-9351; ESSN: 1096-0953 10.1016/j.envres.2016.08.006 |
| spellingShingle | Karami, Ali Omar, Dzolkhifli Lazorchak, James M. Chee, Kong Yap Hashim, Zailina Courtenay, Simon C. A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title | A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title_full | A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title_fullStr | A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title_full_unstemmed | A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title_short | A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor |
| title_sort | comparison of biomarker responses in juvenile diploid and triploid african catfish, clarias gariepinus, exposed to the pesticide butachlor |
| url | http://psasir.upm.edu.my/id/eprint/54825/ http://psasir.upm.edu.my/id/eprint/54825/ http://psasir.upm.edu.my/id/eprint/54825/1/A%20comparison%20of%20biomarker%20responses%20in%20juvenile%20diploid%20and%20triploid%20African%20catfish%2C%20.pdf |