Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion
Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition,...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54799/ http://psasir.upm.edu.my/id/eprint/54799/1/Methicillin-resistant%20Staphylococcus%20aureus%20biofilms%20and%20their%20influence%20on%20bacterial%20adhesion%20and%20cohesion.pdf |
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| author | Dakheel, Khulood Hamid Abdul Rahim, Raha Neela, Vasantha Kumari Al-Obaidi, Jameel R. Tan, Geok Hun Yusoff, Khatijah |
| author_facet | Dakheel, Khulood Hamid Abdul Rahim, Raha Neela, Vasantha Kumari Al-Obaidi, Jameel R. Tan, Geok Hun Yusoff, Khatijah |
| author_sort | Dakheel, Khulood Hamid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype was spa type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested. |
| first_indexed | 2025-11-15T10:41:10Z |
| format | Article |
| id | upm-54799 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:41:10Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-547992018-04-24T07:26:58Z http://psasir.upm.edu.my/id/eprint/54799/ Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion Dakheel, Khulood Hamid Abdul Rahim, Raha Neela, Vasantha Kumari Al-Obaidi, Jameel R. Tan, Geok Hun Yusoff, Khatijah Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype was spa type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54799/1/Methicillin-resistant%20Staphylococcus%20aureus%20biofilms%20and%20their%20influence%20on%20bacterial%20adhesion%20and%20cohesion.pdf Dakheel, Khulood Hamid and Abdul Rahim, Raha and Neela, Vasantha Kumari and Al-Obaidi, Jameel R. and Tan, Geok Hun and Yusoff, Khatijah (2016) Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion. BioMed Research International, 2016. art. no. 4708425. pp. 1-14. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2016/4708425/abs/ 10.1155/2016/4708425 |
| spellingShingle | Dakheel, Khulood Hamid Abdul Rahim, Raha Neela, Vasantha Kumari Al-Obaidi, Jameel R. Tan, Geok Hun Yusoff, Khatijah Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title | Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title_full | Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title_fullStr | Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title_full_unstemmed | Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title_short | Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| title_sort | methicillin-resistant staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion |
| url | http://psasir.upm.edu.my/id/eprint/54799/ http://psasir.upm.edu.my/id/eprint/54799/ http://psasir.upm.edu.my/id/eprint/54799/ http://psasir.upm.edu.my/id/eprint/54799/1/Methicillin-resistant%20Staphylococcus%20aureus%20biofilms%20and%20their%20influence%20on%20bacterial%20adhesion%20and%20cohesion.pdf |