Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways
The objectives of the present study were to determine the mechanisms of antinociceptive effect of methanol extract of Clinacanthus nutans (Acanthaceae) leaves (MECN) using various animal nociceptive models. The antinociceptive activity of orally administered 10% DMSO, 100 mg/kg acetylsalicylic acid...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54798/ http://psasir.upm.edu.my/id/eprint/54798/1/Methanolic%20extract%20of%20Clinacanthus%20nutans%20exerts%20antinociceptive%20activity.pdf |
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| author | Abdul Rahim, Mohammad Hafiz Zakaria, Zainul Amiruddin Mohd Sani, Mohd Hijaz Omar, Maizatul Hasyima Yakob, Yusnita Singh, Manraj Cheema Ching, Siew Mooi Ahmad, Zuraini Abdul Kadir, Arifah |
| author_facet | Abdul Rahim, Mohammad Hafiz Zakaria, Zainul Amiruddin Mohd Sani, Mohd Hijaz Omar, Maizatul Hasyima Yakob, Yusnita Singh, Manraj Cheema Ching, Siew Mooi Ahmad, Zuraini Abdul Kadir, Arifah |
| author_sort | Abdul Rahim, Mohammad Hafiz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objectives of the present study were to determine the mechanisms of antinociceptive effect of methanol extract of Clinacanthus nutans (Acanthaceae) leaves (MECN) using various animal nociceptive models. The antinociceptive activity of orally administered 10% DMSO, 100 mg/kg acetylsalicylic acid (ASA), 5 mg/kg morphine, or MECN (100, 250, and 500 mg/kg) was determined using the acetic acid-induced abdominal constriction (ACT), formalin-induced paw licking (FT), and hot plate tests (HPT). The role of opioid and nitric oxide/cyclic guanosine monophosphate (NO/cGMP) systems was also investigated. The results showed that MECN produced a significant () antinociceptive response in all nociceptive models with the recorded value of 279.3 mg/kg for the ACT, while, for the early and late phases of the FT, the value was >500 mg/kg or 227.7 mg/kg, respectively. This antinociceptive activity was fully antagonized by naloxone (a nonselective opioid antagonist) but was partially reversed by L-arginine (L-arg; a nitric oxide [NO] precursor), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME; an NO synthase inhibitor), or their combinations thereof. In contrast, 1H-[,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ; a soluble guanylyl cyclase inhibitor) enhanced the extract’s antinociception. UHPLC analysis revealed the presence of several flavonoid-based compounds with antinociceptive action. In conclusion, MECN exerted the peripherally and centrally mediated antinociceptive activity via the modulation of the opioid/NO-mediated, but cGMP-independent, systems. |
| first_indexed | 2025-11-15T10:41:10Z |
| format | Article |
| id | upm-54798 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:41:10Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-547982018-04-24T07:21:31Z http://psasir.upm.edu.my/id/eprint/54798/ Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways Abdul Rahim, Mohammad Hafiz Zakaria, Zainul Amiruddin Mohd Sani, Mohd Hijaz Omar, Maizatul Hasyima Yakob, Yusnita Singh, Manraj Cheema Ching, Siew Mooi Ahmad, Zuraini Abdul Kadir, Arifah The objectives of the present study were to determine the mechanisms of antinociceptive effect of methanol extract of Clinacanthus nutans (Acanthaceae) leaves (MECN) using various animal nociceptive models. The antinociceptive activity of orally administered 10% DMSO, 100 mg/kg acetylsalicylic acid (ASA), 5 mg/kg morphine, or MECN (100, 250, and 500 mg/kg) was determined using the acetic acid-induced abdominal constriction (ACT), formalin-induced paw licking (FT), and hot plate tests (HPT). The role of opioid and nitric oxide/cyclic guanosine monophosphate (NO/cGMP) systems was also investigated. The results showed that MECN produced a significant () antinociceptive response in all nociceptive models with the recorded value of 279.3 mg/kg for the ACT, while, for the early and late phases of the FT, the value was >500 mg/kg or 227.7 mg/kg, respectively. This antinociceptive activity was fully antagonized by naloxone (a nonselective opioid antagonist) but was partially reversed by L-arginine (L-arg; a nitric oxide [NO] precursor), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME; an NO synthase inhibitor), or their combinations thereof. In contrast, 1H-[,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ; a soluble guanylyl cyclase inhibitor) enhanced the extract’s antinociception. UHPLC analysis revealed the presence of several flavonoid-based compounds with antinociceptive action. In conclusion, MECN exerted the peripherally and centrally mediated antinociceptive activity via the modulation of the opioid/NO-mediated, but cGMP-independent, systems. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54798/1/Methanolic%20extract%20of%20Clinacanthus%20nutans%20exerts%20antinociceptive%20activity.pdf Abdul Rahim, Mohammad Hafiz and Zakaria, Zainul Amiruddin and Mohd Sani, Mohd Hijaz and Omar, Maizatul Hasyima and Yakob, Yusnita and Singh, Manraj and Cheema and Ching, Siew Mooi and Ahmad, Zuraini and Abdul Kadir, Arifah (2016) Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways. Evidence-Based Complementary and Alternative Medicine, 2016. art. no. 1494981. pp. 1-11. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2016/1494981/abs/ 10.1155/2016/1494981 |
| spellingShingle | Abdul Rahim, Mohammad Hafiz Zakaria, Zainul Amiruddin Mohd Sani, Mohd Hijaz Omar, Maizatul Hasyima Yakob, Yusnita Singh, Manraj Cheema Ching, Siew Mooi Ahmad, Zuraini Abdul Kadir, Arifah Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title | Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title_full | Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title_fullStr | Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title_full_unstemmed | Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title_short | Methanolic extract of Clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cGMP-independent, pathways |
| title_sort | methanolic extract of clinacanthus nutans exerts antinociceptive activity via the opioid/nitric oxide-mediated, but cgmp-independent, pathways |
| url | http://psasir.upm.edu.my/id/eprint/54798/ http://psasir.upm.edu.my/id/eprint/54798/ http://psasir.upm.edu.my/id/eprint/54798/ http://psasir.upm.edu.my/id/eprint/54798/1/Methanolic%20extract%20of%20Clinacanthus%20nutans%20exerts%20antinociceptive%20activity.pdf |