Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia
In this study, we isolated two indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Cellulosimicrobi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54642/ http://psasir.upm.edu.my/id/eprint/54642/1/Isolation%2C%20identification%20and%20diesel-oil%20biodegradation%20capacities%20.pdf |
| _version_ | 1848852599537664000 |
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| author | Nkem, Bruno Martins Halimoon, Normala Md Yusoff, Fatimah Wan Johari, Wan Lufti Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan |
| author_facet | Nkem, Bruno Martins Halimoon, Normala Md Yusoff, Fatimah Wan Johari, Wan Lufti Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan |
| author_sort | Nkem, Bruno Martins |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we isolated two indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source. Results analysed using GC–MS, showed diesel-oil alkanes were degraded an average 64.4% by C. cellulans and 58.1% by A. baumannii with medium optical density reaching 0.967 (C. cellulans) and 1.515 (A. baumannii) in minimal salt media at 32 °C for 10 days. Individual diesel-oil alkanes were degraded between 10%–95.4% by C. cellulans and 0.2%–95.9% by A. baumannii. Both strains utilized diesel-oil for growth. The study suggests both strains are part of indigenous hydrocarbon-degrading bacteria in tarball with potential for bioremediation of oil-polluted marine environment. |
| first_indexed | 2025-11-15T10:40:38Z |
| format | Article |
| id | upm-54642 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:38Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546422018-04-17T07:35:24Z http://psasir.upm.edu.my/id/eprint/54642/ Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia Nkem, Bruno Martins Halimoon, Normala Md Yusoff, Fatimah Wan Johari, Wan Lufti Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan In this study, we isolated two indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source. Results analysed using GC–MS, showed diesel-oil alkanes were degraded an average 64.4% by C. cellulans and 58.1% by A. baumannii with medium optical density reaching 0.967 (C. cellulans) and 1.515 (A. baumannii) in minimal salt media at 32 °C for 10 days. Individual diesel-oil alkanes were degraded between 10%–95.4% by C. cellulans and 0.2%–95.9% by A. baumannii. Both strains utilized diesel-oil for growth. The study suggests both strains are part of indigenous hydrocarbon-degrading bacteria in tarball with potential for bioremediation of oil-polluted marine environment. Elsevier 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54642/1/Isolation%2C%20identification%20and%20diesel-oil%20biodegradation%20capacities%20.pdf Nkem, Bruno Martins and Halimoon, Normala and Md Yusoff, Fatimah and Wan Johari, Wan Lufti and Zakaria, Mohamad Pauzi and Medipally, Srikanth Reddy and Kannan, Narayanan (2016) Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia. Marine Pollution Bulletin, 107 (1). pp. 261-268. ISSN 0025-326X; ESSN: 1879-3363 https://www.sciencedirect.com/science/article/pii/S0025326X16301916 10.1016/j.marpolbul.2016.03.060 |
| spellingShingle | Nkem, Bruno Martins Halimoon, Normala Md Yusoff, Fatimah Wan Johari, Wan Lufti Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title | Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title_full | Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title_fullStr | Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title_full_unstemmed | Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title_short | Isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach, Malaysia |
| title_sort | isolation, identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of cellulosimicrobium cellulans and acinetobacter baumannii from tarball at terengganu beach, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/54642/ http://psasir.upm.edu.my/id/eprint/54642/ http://psasir.upm.edu.my/id/eprint/54642/ http://psasir.upm.edu.my/id/eprint/54642/1/Isolation%2C%20identification%20and%20diesel-oil%20biodegradation%20capacities%20.pdf |