Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318
Purpose: To determine the effect of the secondary metabolites from Penicillium sp. H9318 on cytotoxicity and cell cycle progression. Methods: A yeast PP1 inhibitory screening system was carried out to confirm the presence of anti-PP1c activity in crude acetone extracts of strain H9318. The extracts...
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| Format: | Article |
| Language: | English |
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54638/ http://psasir.upm.edu.my/id/eprint/54638/1/Isolation%20of%20a%20potential%20anticancer%20agent%20with%20protein%20phosphatase%20inhibitory%20activity%20.pdf |
| _version_ | 1848852598965141504 |
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| author | Ooi, S. C. Ho, Coy Choke Seow, Heng Fong |
| author_facet | Ooi, S. C. Ho, Coy Choke Seow, Heng Fong |
| author_sort | Ooi, S. C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Purpose: To determine the effect of the secondary metabolites from Penicillium sp. H9318 on cytotoxicity and cell cycle progression. Methods: A yeast PP1 inhibitory screening system was carried out to confirm the presence of anti-PP1c activity in crude acetone extracts of strain H9318. The extracts were fractionated and identified as Fraction S1 and Citrinin 9318 (CTN9318). Various cancer cell lines were used to test for the toxicity of the crude acetone extracts, Fraction S1 and Citrinin 9318, using MTT viability assay. Results: It was found that a colorectal cancer cell line, HT-29, was susceptible to Fraction S1 and Citrinin 9318. A propidium iodide (PI)-incorporated DNA assay was used to show that there was G2/M arrest in HT-29 by Citrinin 9318. Conclusion: Citrinin 9318 inhibits the viability of HT-29 via mitotic block. The results suggest that Citrinin 9318 is capable of exerting cytotoxicity and mitotic arrest in a colon cancer cell line, HT29. |
| first_indexed | 2025-11-15T10:40:38Z |
| format | Article |
| id | upm-54638 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:38Z |
| publishDate | 2016 |
| publisher | Pharmacotherapy Group, Faculty of Pharmacy, University of Benin |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546382018-04-17T04:10:44Z http://psasir.upm.edu.my/id/eprint/54638/ Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 Ooi, S. C. Ho, Coy Choke Seow, Heng Fong Purpose: To determine the effect of the secondary metabolites from Penicillium sp. H9318 on cytotoxicity and cell cycle progression. Methods: A yeast PP1 inhibitory screening system was carried out to confirm the presence of anti-PP1c activity in crude acetone extracts of strain H9318. The extracts were fractionated and identified as Fraction S1 and Citrinin 9318 (CTN9318). Various cancer cell lines were used to test for the toxicity of the crude acetone extracts, Fraction S1 and Citrinin 9318, using MTT viability assay. Results: It was found that a colorectal cancer cell line, HT-29, was susceptible to Fraction S1 and Citrinin 9318. A propidium iodide (PI)-incorporated DNA assay was used to show that there was G2/M arrest in HT-29 by Citrinin 9318. Conclusion: Citrinin 9318 inhibits the viability of HT-29 via mitotic block. The results suggest that Citrinin 9318 is capable of exerting cytotoxicity and mitotic arrest in a colon cancer cell line, HT29. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54638/1/Isolation%20of%20a%20potential%20anticancer%20agent%20with%20protein%20phosphatase%20inhibitory%20activity%20.pdf Ooi, S. C. and Ho, Coy Choke and Seow, Heng Fong (2016) Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318. Tropical Journal of Pharmaceutical Research, 15 (7). pp. 1423-1429. ISSN 1596-5996; ESSN: 1596-9827 https://www.ajol.info/index.php/tjpr/article/view/141862 10.4314/tjpr.v15i7.10 |
| spellingShingle | Ooi, S. C. Ho, Coy Choke Seow, Heng Fong Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title | Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title_full | Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title_fullStr | Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title_full_unstemmed | Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title_short | Isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived Penicillium sp. strain H9318 |
| title_sort | isolation of a potential anticancer agent with protein phosphatase inhibitory activity from soil-derived penicillium sp. strain h9318 |
| url | http://psasir.upm.edu.my/id/eprint/54638/ http://psasir.upm.edu.my/id/eprint/54638/ http://psasir.upm.edu.my/id/eprint/54638/ http://psasir.upm.edu.my/id/eprint/54638/1/Isolation%20of%20a%20potential%20anticancer%20agent%20with%20protein%20phosphatase%20inhibitory%20activity%20.pdf |