Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolatio...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/54637/ http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf |
| _version_ | 1848852598725017600 |
|---|---|
| author | Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman |
| author_facet | Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman |
| author_sort | Bande, Faruku |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. |
| first_indexed | 2025-11-15T10:40:38Z |
| format | Article |
| id | upm-54637 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:38Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546372018-04-17T04:12:13Z http://psasir.upm.edu.my/id/eprint/54637/ Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf Bande, Faruku and Arshad, Siti Suri and Omar, Abdul Rahman (2016) Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken. Advances in Virology, 2016. art. no. 9058403. pp. 1-4. ISSN 1687-8639; ESSN: 1687-8647 https://www.hindawi.com/journals/av/2016/9058403/abs/ 10.1155/2016/9058403 |
| spellingShingle | Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title | Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title_full | Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title_fullStr | Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title_full_unstemmed | Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title_short | Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken |
| title_sort | isolation and metagenomic identification of avian leukosis virus associated with mortality in broiler chicken |
| url | http://psasir.upm.edu.my/id/eprint/54637/ http://psasir.upm.edu.my/id/eprint/54637/ http://psasir.upm.edu.my/id/eprint/54637/ http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf |