Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequenci...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/54632/ http://psasir.upm.edu.my/id/eprint/54632/1/Isolation%20and%20characterization.pdf |
| _version_ | 1848852597835825152 |
|---|---|
| author | Akita, Hironaga Kimura, Zen‑ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu |
| author_facet | Akita, Hironaga Kimura, Zen‑ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu |
| author_sort | Akita, Hironaga |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation. |
| first_indexed | 2025-11-15T10:40:37Z |
| format | Article |
| id | upm-54632 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:37Z |
| publishDate | 2016 |
| publisher | SpringerOpen |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546322018-04-17T03:06:18Z http://psasir.upm.edu.my/id/eprint/54632/ Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential Akita, Hironaga Kimura, Zen‑ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation. SpringerOpen 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54632/1/Isolation%20and%20characterization.pdf Akita, Hironaga and Kimura, Zen‑ichiro and Mohd Yusoff, Mohd Zulkhairi and Nakashima, Nobutaka and Hoshino, Tamotsu (2016) Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential. SpringerPlus, 5 (596). pp. 1-5. ISSN 2193-1801 https://springerplus.springeropen.com/articles/10.1186/s40064-016-2237-y 10.1186/s40064-016-2237-y |
| spellingShingle | Akita, Hironaga Kimura, Zen‑ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title | Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title_full | Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title_fullStr | Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title_full_unstemmed | Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title_short | Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential |
| title_sort | isolation and characterization of burkholderia sp. strain cca53 exhibiting ligninolytic potential |
| url | http://psasir.upm.edu.my/id/eprint/54632/ http://psasir.upm.edu.my/id/eprint/54632/ http://psasir.upm.edu.my/id/eprint/54632/ http://psasir.upm.edu.my/id/eprint/54632/1/Isolation%20and%20characterization.pdf |