Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil
Heavy metals and organic xenobiotics including dyes are important industrial components with their usage amounting to the millions of tonnes yearly. Their presence in the environment is a serious pollution issue globally. Bioremediation of these pollutants using microbes with multiple detoxification...
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| Format: | Article |
| Language: | English |
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Universidade Federal da Paraiba
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54631/ http://psasir.upm.edu.my/id/eprint/54631/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20azo-dye%20decolorizing%20Serratia%20marcescens%20strain%20Neni-1%20from%20Indonesian%20soil.pdf |
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| author | Gusmanizar, Neni Halmi, Mohd Izuan Effendi Rusnam, M. Abd Rahman, Mohd Fadhil Shukor, Mohd Shukri Azmi, Nina Suhaity Shukor, Mohd Yunus |
| author_facet | Gusmanizar, Neni Halmi, Mohd Izuan Effendi Rusnam, M. Abd Rahman, Mohd Fadhil Shukor, Mohd Shukri Azmi, Nina Suhaity Shukor, Mohd Yunus |
| author_sort | Gusmanizar, Neni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Heavy metals and organic xenobiotics including dyes are important industrial components with their usage amounting to the millions of tonnes yearly. Their presence in the environment is a serious pollution issue globally. Bioremediation of these pollutants using microbes with multiple detoxification capacity is constantly being sought. In this work we screen the ability of a molybdenum-reducing bacterium isolated from contaminated soil to decolorize various azo and triphenyl methane dyes. The bacterium reduces molybdate to molybdenum blue (Mo-blue) optimally at pH 6.0, and temperatures of between 25 and 40oC. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, trehalose, maltose, d-sorbitol, d-mannitol, d-mannose, myo-inositol, glycerol and salicin in descending order. Other requirements include a phosphate concentration of between 5.0 and 7.5 mM and a molybdate concentration between 10 and 20 mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited bycopper, silver and mercury at 2 ppm by 43.8%, 42.3% and 41.7%, respectively. We screen for the ability of the bacterium to decolorize various dyes. The bacterium was able to decolorize the dye Congo Red. Biochemical analysis resulted in a tentative identification of the bacterium as Serratia marcescens strain Neni-1. The ability of this bacterium to detoxify molybdenum and decolorize azo dye makes this bacteriuman important tool for bioremediation. |
| first_indexed | 2025-11-15T10:40:37Z |
| format | Article |
| id | upm-54631 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:37Z |
| publishDate | 2016 |
| publisher | Universidade Federal da Paraiba |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546312018-04-17T02:57:54Z http://psasir.upm.edu.my/id/eprint/54631/ Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil Gusmanizar, Neni Halmi, Mohd Izuan Effendi Rusnam, M. Abd Rahman, Mohd Fadhil Shukor, Mohd Shukri Azmi, Nina Suhaity Shukor, Mohd Yunus Heavy metals and organic xenobiotics including dyes are important industrial components with their usage amounting to the millions of tonnes yearly. Their presence in the environment is a serious pollution issue globally. Bioremediation of these pollutants using microbes with multiple detoxification capacity is constantly being sought. In this work we screen the ability of a molybdenum-reducing bacterium isolated from contaminated soil to decolorize various azo and triphenyl methane dyes. The bacterium reduces molybdate to molybdenum blue (Mo-blue) optimally at pH 6.0, and temperatures of between 25 and 40oC. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, trehalose, maltose, d-sorbitol, d-mannitol, d-mannose, myo-inositol, glycerol and salicin in descending order. Other requirements include a phosphate concentration of between 5.0 and 7.5 mM and a molybdate concentration between 10 and 20 mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited bycopper, silver and mercury at 2 ppm by 43.8%, 42.3% and 41.7%, respectively. We screen for the ability of the bacterium to decolorize various dyes. The bacterium was able to decolorize the dye Congo Red. Biochemical analysis resulted in a tentative identification of the bacterium as Serratia marcescens strain Neni-1. The ability of this bacterium to detoxify molybdenum and decolorize azo dye makes this bacteriuman important tool for bioremediation. Universidade Federal da Paraiba 2016 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/54631/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20azo-dye%20decolorizing%20Serratia%20marcescens%20strain%20Neni-1%20from%20Indonesian%20soil.pdf Gusmanizar, Neni and Halmi, Mohd Izuan Effendi and Rusnam, M. and Abd Rahman, Mohd Fadhil and Shukor, Mohd Shukri and Azmi, Nina Suhaity and Shukor, Mohd Yunus (2016) Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil. Journal of Urban and Environmental Engineering, 10 (1). pp. 113-123. ISSN 1982-3932 http://www.jstor.org/stable/26240818?seq=1#page_scan_tab_contents 10.4090/juee.2016.v10n1.113123 |
| spellingShingle | Gusmanizar, Neni Halmi, Mohd Izuan Effendi Rusnam, M. Abd Rahman, Mohd Fadhil Shukor, Mohd Shukri Azmi, Nina Suhaity Shukor, Mohd Yunus Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title | Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title_full | Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title_fullStr | Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title_full_unstemmed | Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title_short | Isolation and characterization of a molybdenum-reducing and azo-dye decolorizing Serratia marcescens strain Neni-1 from Indonesian soil |
| title_sort | isolation and characterization of a molybdenum-reducing and azo-dye decolorizing serratia marcescens strain neni-1 from indonesian soil |
| url | http://psasir.upm.edu.my/id/eprint/54631/ http://psasir.upm.edu.my/id/eprint/54631/ http://psasir.upm.edu.my/id/eprint/54631/ http://psasir.upm.edu.my/id/eprint/54631/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20azo-dye%20decolorizing%20Serratia%20marcescens%20strain%20Neni-1%20from%20Indonesian%20soil.pdf |