In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation
Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engin...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Conscientia Beam
2016
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/54621/ http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf |
| _version_ | 1848852595355942912 |
|---|---|
| author | Teng, Suk Kuan Abd. Aziz, Nor Azwady Mustafa, Muskhazli Laboh, Rozeita Ismail, Intan Safinar Devi, Sujithra |
| author_facet | Teng, Suk Kuan Abd. Aziz, Nor Azwady Mustafa, Muskhazli Laboh, Rozeita Ismail, Intan Safinar Devi, Sujithra |
| author_sort | Teng, Suk Kuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engineers that modify soil properties that may favor or suppress different microbial populations. In the study, the effect of endogeic earthworm, Pontoscolex corethrurus on blood disease bacterium (BDB) was evaluated through in-vitro study. Earthworm casts, mucus and worm-worked soil collected were inoculated onto tetrazolium chloride (TZC) medium seeded with BDB at different concentrations. Molecular identification using 16S rRNA primers was then performed to identify the microbial isolates that inhibited BDB. Phylogenetic tree was constructed to determine the similarity among the isolates with Pseudomonas sp. sequences deposited in the GenBank. The results showed that bacterial colonies from earthworm mucus inhibited BDB at 103 and 105 cell/ml. However, no inhibition was observed at higher BDB concentration (107 cell/ml). Similarly, earthworm cast and worm-worked soil did not show inhibition towards BDB. Bacterial isolates obtained from earthworm mucus were classified into the genus Pseudomonas. The study suggested the potential role of P. corethrurus in enhancing the growth of beneficial microorganism in remediating blood disease. |
| first_indexed | 2025-11-15T10:40:34Z |
| format | Article |
| id | upm-54621 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:34Z |
| publishDate | 2016 |
| publisher | Conscientia Beam |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546212018-04-05T02:52:24Z http://psasir.upm.edu.my/id/eprint/54621/ In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation Teng, Suk Kuan Abd. Aziz, Nor Azwady Mustafa, Muskhazli Laboh, Rozeita Ismail, Intan Safinar Devi, Sujithra Blood disease is a wilt disease that greatly affects the banana cultivation. Besides chemicals, the utilization of natural soil-biological entity may provide an alternative in searching for effective and environmental friendly approach against the disease. Endogeic earthworms are known as soil-engineers that modify soil properties that may favor or suppress different microbial populations. In the study, the effect of endogeic earthworm, Pontoscolex corethrurus on blood disease bacterium (BDB) was evaluated through in-vitro study. Earthworm casts, mucus and worm-worked soil collected were inoculated onto tetrazolium chloride (TZC) medium seeded with BDB at different concentrations. Molecular identification using 16S rRNA primers was then performed to identify the microbial isolates that inhibited BDB. Phylogenetic tree was constructed to determine the similarity among the isolates with Pseudomonas sp. sequences deposited in the GenBank. The results showed that bacterial colonies from earthworm mucus inhibited BDB at 103 and 105 cell/ml. However, no inhibition was observed at higher BDB concentration (107 cell/ml). Similarly, earthworm cast and worm-worked soil did not show inhibition towards BDB. Bacterial isolates obtained from earthworm mucus were classified into the genus Pseudomonas. The study suggested the potential role of P. corethrurus in enhancing the growth of beneficial microorganism in remediating blood disease. Conscientia Beam 2016-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf Teng, Suk Kuan and Abd. Aziz, Nor Azwady and Mustafa, Muskhazli and Laboh, Rozeita and Ismail, Intan Safinar and Devi, Sujithra (2016) In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation. The Asia Journal of Applied Microbiology, 3 (1). pp. 1-11. ISSN 2409-2177; ESSN: 2313-8157 https://econpapers.repec.org/article/pkptajoam/2016_3ap_3a1-11.htm Banana; Blood disease bacterium (BDB); Cast; Mucus; Pontoscolex corethrurus; Pseudomonas Sp. 10.18488/journal.33/2016.3.1/33.1.1.11 |
| spellingShingle | Banana; Blood disease bacterium (BDB); Cast; Mucus; Pontoscolex corethrurus; Pseudomonas Sp. Teng, Suk Kuan Abd. Aziz, Nor Azwady Mustafa, Muskhazli Laboh, Rozeita Ismail, Intan Safinar Devi, Sujithra In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title | In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title_full | In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title_fullStr | In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title_full_unstemmed | In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title_short | In-vitro study on the effect of endogeic earthworm on blood disease bacterium (BDB) in banana: a preliminary observation |
| title_sort | in-vitro study on the effect of endogeic earthworm on blood disease bacterium (bdb) in banana: a preliminary observation |
| topic | Banana; Blood disease bacterium (BDB); Cast; Mucus; Pontoscolex corethrurus; Pseudomonas Sp. |
| url | http://psasir.upm.edu.my/id/eprint/54621/ http://psasir.upm.edu.my/id/eprint/54621/ http://psasir.upm.edu.my/id/eprint/54621/ http://psasir.upm.edu.my/id/eprint/54621/1/In-vitro%20study%20on%20the%20effect%20of%20endogeic%20earthworm%20on%20blood%20disease%20bacterium%20.pdf |