In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions

The high nutrition value of kenaf seed oil has good potential to be used as functional foods or nutraceutical products. Kenaf seed oil-in-water nanoemulsions stabilised by ternary emulsifier mixtures, namely sodium caseinate, gum Arabic and Tween 20 were produced by using high pressure homogeniser....

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Main Authors: Cheong, Ai Mun, Tan, Chin Ping, Nyam, Kar Lin
Format: Article
Language:English
Published: Elsevier 2016
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/54620/
http://psasir.upm.edu.my/id/eprint/54620/1/In-vitro%20gastrointestinal%20digestion%20of%20kenaf%20seed%20oil-in-water%20nanoemulsions.pdf
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author Cheong, Ai Mun
Tan, Chin Ping
Nyam, Kar Lin
author_facet Cheong, Ai Mun
Tan, Chin Ping
Nyam, Kar Lin
author_sort Cheong, Ai Mun
building UPM Institutional Repository
collection Online Access
description The high nutrition value of kenaf seed oil has good potential to be used as functional foods or nutraceutical products. Kenaf seed oil-in-water nanoemulsions stabilised by ternary emulsifier mixtures, namely sodium caseinate, gum Arabic and Tween 20 were produced by using high pressure homogeniser. A two-stage in-vitro model was employed to investigate the bioaccessibility of bioactive compounds that is naturally present in the kenaf seed oil-in-water nanoemulsions. The changes in the antioxidants properties before and after in-vitro digestion and the structural changes during digestion were also evaluated. By comparing the digested and undigested nanoemulsions, the digested nanoemulsions had increased the total phenolic content by 71% and tocopherol content by 230%. However, 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radical scavenging activity was decreased by 34% and phytosterols content was decreased by 39%. The amount of free fatty acids (FFA) released from gastric digested nanoemulsions during 120 min of intestinal digestion was 247.7 μmol/mL. This high release of FFA indicates good lipid digestion, which is the preliminary step for releasing and absorption of lipophilic bioactive in the small intestine. This study provides useful insights into the changes of kenaf seed oil-in-water nanoemulsions during gastrointestinal digestion.
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spelling upm-546202018-04-05T02:40:49Z http://psasir.upm.edu.my/id/eprint/54620/ In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions Cheong, Ai Mun Tan, Chin Ping Nyam, Kar Lin The high nutrition value of kenaf seed oil has good potential to be used as functional foods or nutraceutical products. Kenaf seed oil-in-water nanoemulsions stabilised by ternary emulsifier mixtures, namely sodium caseinate, gum Arabic and Tween 20 were produced by using high pressure homogeniser. A two-stage in-vitro model was employed to investigate the bioaccessibility of bioactive compounds that is naturally present in the kenaf seed oil-in-water nanoemulsions. The changes in the antioxidants properties before and after in-vitro digestion and the structural changes during digestion were also evaluated. By comparing the digested and undigested nanoemulsions, the digested nanoemulsions had increased the total phenolic content by 71% and tocopherol content by 230%. However, 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radical scavenging activity was decreased by 34% and phytosterols content was decreased by 39%. The amount of free fatty acids (FFA) released from gastric digested nanoemulsions during 120 min of intestinal digestion was 247.7 μmol/mL. This high release of FFA indicates good lipid digestion, which is the preliminary step for releasing and absorption of lipophilic bioactive in the small intestine. This study provides useful insights into the changes of kenaf seed oil-in-water nanoemulsions during gastrointestinal digestion. Elsevier 2016-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54620/1/In-vitro%20gastrointestinal%20digestion%20of%20kenaf%20seed%20oil-in-water%20nanoemulsions.pdf Cheong, Ai Mun and Tan, Chin Ping and Nyam, Kar Lin (2016) In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions. Industrial Crops and Products, 87. pp. 1-8. ISSN 0926-6690; ESSN: 1872-633X https://www.sciencedirect.com/science/article/pii/S0926669016302357 Kenaf (Hibiscus cannabinus L.) seed oil; High pressure homogenizer; In-vitro digestion; Bioaccessibility; Antioxidant activity; Confocal microscopy 10.1016/j.indcrop.2016.04.019
spellingShingle Kenaf (Hibiscus cannabinus L.) seed oil; High pressure homogenizer; In-vitro digestion; Bioaccessibility; Antioxidant activity; Confocal microscopy
Cheong, Ai Mun
Tan, Chin Ping
Nyam, Kar Lin
In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title_full In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title_fullStr In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title_full_unstemmed In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title_short In-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
title_sort in-vitro gastrointestinal digestion of kenaf seed oil-in-water nanoemulsions
topic Kenaf (Hibiscus cannabinus L.) seed oil; High pressure homogenizer; In-vitro digestion; Bioaccessibility; Antioxidant activity; Confocal microscopy
url http://psasir.upm.edu.my/id/eprint/54620/
http://psasir.upm.edu.my/id/eprint/54620/
http://psasir.upm.edu.my/id/eprint/54620/
http://psasir.upm.edu.my/id/eprint/54620/1/In-vitro%20gastrointestinal%20digestion%20of%20kenaf%20seed%20oil-in-water%20nanoemulsions.pdf