In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats
Erythroxylum cuneatum has been traditionally proven to possess beneficial properties in treating drug addiction and other illness. Due to less information on this plant, this elusive plant was investigated further to evaluate the in vivo toxicity profile of the plant and to investigate the effect of E...
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| Format: | Article |
| Language: | English |
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2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54543/ http://psasir.upm.edu.my/id/eprint/54543/1/In%20vivo%20toxicity%20study%20of%20Erythroxylum%20cuneatum%20leaves%20extract%20.pdf |
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| author | Shahril, Mohamad Syahmi Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Adenan, Mohd Ilham Amar Jamil, Mohd Fadzly Mat Taib, Che Norma Suliman, Noor Azuin Ahmad Zaki, Muhammad Amin |
| author_facet | Shahril, Mohamad Syahmi Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Adenan, Mohd Ilham Amar Jamil, Mohd Fadzly Mat Taib, Che Norma Suliman, Noor Azuin Ahmad Zaki, Muhammad Amin |
| author_sort | Shahril, Mohamad Syahmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Erythroxylum cuneatum has been traditionally proven to possess beneficial properties in treating drug addiction and other illness. Due to less information on this plant, this elusive plant was investigated further to evaluate the in vivo toxicity profile of the plant and to investigate the effect of E. cuneatum on cognitive performance in rats. Two different extracts were produced from the leaves of E. cuneatum which were aqueous and alkaloid extracts. Acute in vivo toxicity test was evaluated in ICR mice to determine their medium lethal dose 50 value. In the in-vivo toxicity study, aqueous extract showed the almost similar toxic effect as alkaloid extract which was 416.86 mg/kg for alkaloid extract and 316.23 mg/kg for aqueous extract. These findings suggesting that aqueous and alkaloid extracts showed toxic effects at the high dose, thus safe at a low dose. Working memory task using novel object discrimination test (NOD) was performed for the determination of neurobehavioral profiles. In the NOD test, alkaloid-treated rats did not show any significant discrimination between the familiar and novel object (P > 0.05); thus it can be interpreted as not induce a memory defi cit. It can also be postulated that the extract has no effect on memory and learning neither improvises nor impairs the cognitive function. In conclusion, since E. cuneatum does not show any impairment on cognitive, its pharmacological properties could be further investigated without significant changes in cognitive performance. |
| first_indexed | 2025-11-15T10:40:14Z |
| format | Article |
| id | upm-54543 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:14Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-545432018-03-27T08:52:14Z http://psasir.upm.edu.my/id/eprint/54543/ In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats Shahril, Mohamad Syahmi Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Adenan, Mohd Ilham Amar Jamil, Mohd Fadzly Mat Taib, Che Norma Suliman, Noor Azuin Ahmad Zaki, Muhammad Amin Erythroxylum cuneatum has been traditionally proven to possess beneficial properties in treating drug addiction and other illness. Due to less information on this plant, this elusive plant was investigated further to evaluate the in vivo toxicity profile of the plant and to investigate the effect of E. cuneatum on cognitive performance in rats. Two different extracts were produced from the leaves of E. cuneatum which were aqueous and alkaloid extracts. Acute in vivo toxicity test was evaluated in ICR mice to determine their medium lethal dose 50 value. In the in-vivo toxicity study, aqueous extract showed the almost similar toxic effect as alkaloid extract which was 416.86 mg/kg for alkaloid extract and 316.23 mg/kg for aqueous extract. These findings suggesting that aqueous and alkaloid extracts showed toxic effects at the high dose, thus safe at a low dose. Working memory task using novel object discrimination test (NOD) was performed for the determination of neurobehavioral profiles. In the NOD test, alkaloid-treated rats did not show any significant discrimination between the familiar and novel object (P > 0.05); thus it can be interpreted as not induce a memory defi cit. It can also be postulated that the extract has no effect on memory and learning neither improvises nor impairs the cognitive function. In conclusion, since E. cuneatum does not show any impairment on cognitive, its pharmacological properties could be further investigated without significant changes in cognitive performance. 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54543/1/In%20vivo%20toxicity%20study%20of%20Erythroxylum%20cuneatum%20leaves%20extract%20.pdf Shahril, Mohamad Syahmi and Mohd Moklas, Mohamad Aris and Baharuldin, Mohamad Taufik Hidayat and Basir, Rusliza and Adenan, Mohd Ilham and Amar Jamil, Mohd Fadzly and Mat Taib, Che Norma and Suliman, Noor Azuin and Ahmad Zaki, Muhammad Amin (2016) In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats. Journal of Pharmacological and Toxicological Investigations, 2 (2). pp. 10-13. Erythroxylum cuneatum; Medium lethal dose; Acute toxicity study; Novel object discrimination test; Working memory |
| spellingShingle | Erythroxylum cuneatum; Medium lethal dose; Acute toxicity study; Novel object discrimination test; Working memory Shahril, Mohamad Syahmi Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Adenan, Mohd Ilham Amar Jamil, Mohd Fadzly Mat Taib, Che Norma Suliman, Noor Azuin Ahmad Zaki, Muhammad Amin In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title | In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title_full | In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title_fullStr | In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title_full_unstemmed | In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title_short | In vivo toxicity study of Erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| title_sort | in vivo toxicity study of erythroxylum cuneatum leaves extract and its effects on working memory of rats |
| topic | Erythroxylum cuneatum; Medium lethal dose; Acute toxicity study; Novel object discrimination test; Working memory |
| url | http://psasir.upm.edu.my/id/eprint/54543/ http://psasir.upm.edu.my/id/eprint/54543/1/In%20vivo%20toxicity%20study%20of%20Erythroxylum%20cuneatum%20leaves%20extract%20.pdf |