Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol
Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPC...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Analytical Sciences Society
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54496/ http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf |
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| author | Abd Manan, Fariza Aina Abdullah, Jaafar Nazri, Nur Nadziera Abd Malik, Izyan Nadira Yusof, Nor Azah Ahmad, Ishak |
| author_facet | Abd Manan, Fariza Aina Abdullah, Jaafar Nazri, Nur Nadziera Abd Malik, Izyan Nadira Yusof, Nor Azah Ahmad, Ishak |
| author_sort | Abd Manan, Fariza Aina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39 – 7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%. |
| first_indexed | 2025-11-15T10:40:01Z |
| format | Article |
| id | upm-54496 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:01Z |
| publishDate | 2016 |
| publisher | Malaysian Analytical Sciences Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-544962018-03-21T03:22:07Z http://psasir.upm.edu.my/id/eprint/54496/ Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol Abd Manan, Fariza Aina Abdullah, Jaafar Nazri, Nur Nadziera Abd Malik, Izyan Nadira Yusof, Nor Azah Ahmad, Ishak Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39 – 7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%. Malaysian Analytical Sciences Society 2016-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf Abd Manan, Fariza Aina and Abdullah, Jaafar and Nazri, Nur Nadziera and Abd Malik, Izyan Nadira and Yusof, Nor Azah and Ahmad, Ishak (2016) Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol. Malaysian Journal of Analytical Sciences, 20 (5). pp. 978-985. ISSN 1394-2506 http://www.ukm.my/mjas/mjas2018/ Nanocrystalline cellulose; Nanocomposite; Immobilization; Eectrochemical; Phenol 10.17576/mjas-2016-2005-02 |
| spellingShingle | Nanocrystalline cellulose; Nanocomposite; Immobilization; Eectrochemical; Phenol Abd Manan, Fariza Aina Abdullah, Jaafar Nazri, Nur Nadziera Abd Malik, Izyan Nadira Yusof, Nor Azah Ahmad, Ishak Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title | Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title_full | Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title_fullStr | Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title_full_unstemmed | Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title_short | Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| title_sort | immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol |
| topic | Nanocrystalline cellulose; Nanocomposite; Immobilization; Eectrochemical; Phenol |
| url | http://psasir.upm.edu.my/id/eprint/54496/ http://psasir.upm.edu.my/id/eprint/54496/ http://psasir.upm.edu.my/id/eprint/54496/ http://psasir.upm.edu.my/id/eprint/54496/1/Immobilization%20of%20tyrosinase%20in%20nanocrystalline%20cellulose.pdf |